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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Alkaline phosphatase (SPBC14F5.13c), partial CSB-YP001633SXV1
CSB-EP001633SXV1
CSB-BP001633SXV1
CSB-MP001633SXV1
CSB-EP001633SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 10 (taf10) CSB-YP023077SXV
CSB-EP023077SXV
CSB-BP023077SXV
CSB-MP023077SXV
CSB-EP023077SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cystinosin (CTNS), partial CSB-YP006174HU1
CSB-EP006174HU1
CSB-BP006174HU1
CSB-MP006174HU1
CSB-EP006174HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Katanin p80 WD40-containing subunit B1 (KATNB1), partial CSB-YP011993FOT
CSB-EP011993FOT
CSB-BP011993FOT
CSB-MP011993FOT
CSB-EP011993FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic translation initiation factor 3 subunit E (eif-3.E) CSB-YP007534CXY
CSB-EP007534CXY
CSB-BP007534CXY
CSB-MP007534CXY
CSB-EP007534CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant mRNA-decapping enzyme 2 (dcap-2), partial CSB-YP006557CXY
CSB-EP006557CXY
CSB-BP006557CXY
CSB-MP006557CXY
CSB-EP006557CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiri sciureus Membrane cofactor protein (CD46) CSB-YP004939SWD
CSB-EP004939SWD
CSB-BP004939SWD
CSB-MP004939SWD
CSB-EP004939SWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DIG
CSB-EP015076DIG
CSB-BP015076DIG
CSB-MP015076DIG
CSB-EP015076DIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas formosa NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077BZB
CSB-EP015077BZB
CSB-BP015077BZB
CSB-MP015077BZB
CSB-EP015077BZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea mariana 40S ribosomal protein S15 (RPS15) CSB-YP020372EVM
CSB-EP020372EVM
CSB-BP020372EVM
CSB-MP020372EVM
CSB-EP020372EVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zantedeschia aethiopica Superoxide dismutase [Cu-Zn] (SODCC) CSB-YP022397ZAV
CSB-EP022397ZAV
CSB-BP022397ZAV
CSB-MP022397ZAV
CSB-EP022397ZAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Flap endonuclease 1 (FEN1) CSB-YP008585DOA
CSB-EP008585DOA
CSB-BP008585DOA
CSB-MP008585DOA
CSB-EP008585DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helianthus annuus S-adenosylmethionine decarboxylase proenzyme (SAMDC) CSB-YP001658HUO
CSB-EP001658HUO
CSB-BP001658HUO
CSB-MP001658HUO
CSB-EP001658HUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum 60S ribosomal protein L24 (RPL24) CSB-YP020202DTI
CSB-EP020202DTI
CSB-BP020202DTI
CSB-MP020202DTI
CSB-EP020202DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hevea brasiliensis Profilin-1 CSB-YP017824HWI
CSB-EP017824HWI
CSB-BP017824HWI
CSB-MP017824HWI
CSB-EP017824HWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Striga hermonthica Non-specific lipid-transfer protein CSB-YP020856FOS
CSB-EP020856FOS
CSB-BP020856FOS
CSB-MP020856FOS
CSB-EP020856FOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP017878TPR1
CSB-EP017878TPR1
CSB-BP017878TPR1
CSB-MP017878TPR1
CSB-EP017878TPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Porphobilinogen deaminase (hemC) CSB-YP010524DNV
CSB-EP010524DNV
CSB-BP010524DNV
CSB-MP010524DNV
CSB-EP010524DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP010706DNV
CSB-EP010706DNV
CSB-BP010706DNV
CSB-MP010706DNV
CSB-EP010706DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Elongation factor Ts (tsf) CSB-YP025124DNV
CSB-EP025124DNV
CSB-BP025124DNV
CSB-MP025124DNV
CSB-EP025124DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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