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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Protein Wnt-11 (wnt11) CSB-YP026131DIL
CSB-EP026131DIL
CSB-BP026131DIL
CSB-MP026131DIL
CSB-EP026131DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus DNA topoisomerase 1 (topA) CSB-YP024056FHW
CSB-EP024056FHW
CSB-BP024056FHW
CSB-MP024056FHW
CSB-EP024056FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP024102MRN
CSB-EP024102MRN
CSB-BP024102MRN
CSB-MP024102MRN
CSB-EP024102MRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Signal recognition particle 19 kDa protein (srp19) CSB-YP022674FHX
CSB-EP022674FHX
CSB-BP022674FHX
CSB-MP022674FHX
CSB-EP022674FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cell division control protein 45 homolog (sna41), partial CSB-YP005020SXV
CSB-EP005020SXV
CSB-BP005020SXV
CSB-MP005020SXV
CSB-EP005020SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agaricus bisporus Aldehyde dehydrogenase (aldA) CSB-YP001572AYJ
CSB-EP001572AYJ
CSB-BP001572AYJ
CSB-MP001572AYJ
CSB-EP001572AYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Origin recognition complex subunit 1 (ORC1), partial CSB-YP017231CZF
CSB-EP017231CZF
CSB-BP017231CZF
CSB-MP017231CZF
CSB-EP017231CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe N-alpha-acetyltransferase 30 (MAK3) CSB-YP015467SXV
CSB-EP015467SXV
CSB-BP015467SXV
CSB-MP015467SXV
CSB-EP015467SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S29 (rps29) CSB-YP020432SXV
CSB-EP020432SXV
CSB-BP020432SXV
CSB-MP020432SXV
CSB-EP020432SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Bromodomain-containing protein 1 (bdc1) CSB-YP002799SXV
CSB-EP002799SXV
CSB-BP002799SXV
CSB-MP002799SXV
CSB-EP002799SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 6 (taf6) CSB-YP023095SXV
CSB-EP023095SXV
CSB-BP023095SXV
CSB-MP023095SXV
CSB-EP023095SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 3-keto-steroid reductase (erg27) CSB-YP010776SXV
CSB-EP010776SXV
CSB-BP010776SXV
CSB-MP010776SXV
CSB-EP010776SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe KRR1 small subunit processome component homolog (mis3) CSB-YP012502SXV
CSB-EP012502SXV
CSB-BP012502SXV
CSB-MP012502SXV
CSB-EP012502SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial inner membrane protease subunit 1 (imp1) CSB-YP011688SXV
CSB-EP011688SXV
CSB-BP011688SXV
CSB-MP011688SXV
CSB-EP011688SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable peptidyl-tRNA hydrolase (SPBC2D10.15c) CSB-YP019071SXV
CSB-EP019071SXV
CSB-BP019071SXV
CSB-MP019071SXV
CSB-EP019071SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase III subunit rpc5 (rpc37) CSB-YP018347SXV
CSB-EP018347SXV
CSB-BP018347SXV
CSB-MP018347SXV
CSB-EP018347SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S20 (rps20) CSB-YP020394SXV
CSB-EP020394SXV
CSB-BP020394SXV
CSB-MP020394SXV
CSB-EP020394SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Diphthine synthase (dph5) CSB-YP007133SXV
CSB-EP007133SXV
CSB-BP007133SXV
CSB-MP007133SXV
CSB-EP007133SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA polymerase alpha subunit B (pol12), partial CSB-YP018301SXV
CSB-EP018301SXV
CSB-BP018301SXV
CSB-MP018301SXV
CSB-EP018301SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Vacuolar protein-sorting-associated protein 25 (vps25) CSB-YP025897SXV
CSB-EP025897SXV
CSB-BP025897SXV
CSB-MP025897SXV
CSB-EP025897SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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