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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Pre-mRNA-processing factor 39 (prp39), partial CSB-YP018767SXV
CSB-EP018767SXV
CSB-BP018767SXV
CSB-MP018767SXV
CSB-EP018767SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Pterin-4-alpha-carbinolamine dehydratase (Pcd) CSB-YP017514DLU
CSB-EP017514DLU
CSB-BP017514DLU
CSB-MP017514DLU
CSB-EP017514DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spodoptera frugiperda Casein kinase II subunit beta CSB-YP006075FKQ
CSB-EP006075FKQ
CSB-BP006075FKQ
CSB-MP006075FKQ
CSB-EP006075FKQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum T-complex protein 1 subunit eta (MAL3P3.6, PFC0350c), partial CSB-YP004871EWP
CSB-EP004871EWP
CSB-BP004871EWP
CSB-MP004871EWP
CSB-EP004871EWP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Frizzled-3 (fz3), partial CSB-YP009106DLU1
CSB-EP009106DLU1
CSB-BP009106DLU1
CSB-MP009106DLU1
CSB-EP009106DLU1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Galactoside 2-alpha-L-fucosyltransferase 2 (FUT2), partial CSB-YP009076EQV1
CSB-EP009076EQV1
CSB-BP009076EQV1
CSB-MP009076EQV1
CSB-EP009076EQV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erinaceus europaeus Aquaporin-2 (AQP2), partial CSB-YP001962EMQ1
CSB-EP001962EMQ1
CSB-BP001962EMQ1
CSB-MP001962EMQ1
CSB-EP001962EMQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Vasopressin V2 receptor (AVPR2), partial CSB-YP002470DO1
CSB-EP002470DO1
CSB-BP002470DO1
CSB-MP002470DO1
CSB-EP002470DO1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Interferon beta (IFNB1) CSB-YP011048MOV
CSB-EP011048MOV
CSB-BP011048MOV
CSB-MP011048MOV
CSB-EP011048MOV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DQG
CSB-EP015076DQG
CSB-BP015076DQG
CSB-MP015076DQG
CSB-EP015076DQG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081DQG
CSB-EP015081DQG
CSB-BP015081DQG
CSB-MP015081DQG
CSB-EP015081DQG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cebus apella NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DQU
CSB-EP015076DQU
CSB-BP015076DQU
CSB-MP015076DQU
CSB-EP015076DQU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamandua tetradactyla NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076TLB
CSB-EP015076TLB
CSB-BP015076TLB
CSB-MP015076TLB
CSB-EP015076TLB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076MUE
CSB-EP015076MUE
CSB-BP015076MUE
CSB-MP015076MUE
CSB-EP015076MUE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophilus harrisii NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076SXI
CSB-EP015076SXI
CSB-BP015076SXI
CSB-MP015076SXI
CSB-EP015076SXI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovis dalli Cytochrome b (MT-CYB), partial CSB-YP015075OFW
CSB-EP015075OFW
CSB-BP015075OFW
CSB-MP015075OFW
CSB-EP015075OFW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capra sibirica Cytochrome b (MT-CYB), partial CSB-YP015075DQA
CSB-EP015075DQA
CSB-BP015075DQA
CSB-MP015075DQA
CSB-EP015075DQA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capra caucasica Cytochrome b (MT-CYB), partial CSB-YP015075CZW
CSB-EP015075CZW
CSB-BP015075CZW
CSB-MP015075CZW
CSB-EP015075CZW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lontra felina Cytochrome b (MT-CYB), partial CSB-YP015075LQN
CSB-EP015075LQN
CSB-BP015075LQN
CSB-MP015075LQN
CSB-EP015075LQN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Martes americana Cytochrome b (MT-CYB), partial CSB-YP015075MQH
CSB-EP015075MQH
CSB-BP015075MQH
CSB-MP015075MQH
CSB-EP015075MQH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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