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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Carassius auratus Proteasome subunit alpha type-2 (psma2) CSB-YP018866DQG
CSB-EP018866DQG
CSB-BP018866DQG
CSB-MP018866DQG
CSB-EP018866DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcription factor COE3 (coe3), partial CSB-YP007358XBE
CSB-EP007358XBE
CSB-BP007358XBE
CSB-MP007358XBE
CSB-EP007358XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Paired box protein Pax-6 (pax6) CSB-YP017492OFH
CSB-EP017492OFH
CSB-BP017492OFH
CSB-MP017492OFH
CSB-EP017492OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788FHY
CSB-EP013788FHY
CSB-BP013788FHY
CSB-MP013788FHY
CSB-EP013788FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S27 (rps27) CSB-YP020419SXV
CSB-EP020419SXV
CSB-BP020419SXV
CSB-MP020419SXV
CSB-EP020419SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe T-complex protein 1 subunit gamma (cct3), partial CSB-YP004857SXV
CSB-EP004857SXV
CSB-BP004857SXV
CSB-MP004857SXV
CSB-EP004857SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe NEDD8-conjugating enzyme ubc12 (ubc12) CSB-YP025468SXV
CSB-EP025468SXV
CSB-BP025468SXV
CSB-MP025468SXV
CSB-EP025468SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe NADH-cytochrome b5 reductase 1 (cbr1), partial CSB-YP006318SXV1
CSB-EP006318SXV1
CSB-BP006318SXV1
CSB-MP006318SXV1
CSB-EP006318SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase II subunit RPB9 (rpb9) CSB-YP018336SXV
CSB-EP018336SXV
CSB-BP018336SXV
CSB-MP018336SXV
CSB-EP018336SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM9 (TIM9) CSB-YP023559SVG
CSB-EP023559SVG
CSB-BP023559SVG
CSB-MP023559SVG
CSB-EP023559SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA primase large subunit (pri2) CSB-YP018681SXV
CSB-EP018681SXV
CSB-BP018681SXV
CSB-MP018681SXV
CSB-EP018681SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase II subunit rpb4 (rpb4) CSB-YP018331SXV
CSB-EP018331SXV
CSB-BP018331SXV
CSB-MP018331SXV
CSB-EP018331SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe mRNA cap guanine-N7 methyltransferase (pcm1) CSB-YP019902SXV
CSB-EP019902SXV
CSB-BP019902SXV
CSB-MP019902SXV
CSB-EP019902SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor IIA subunit 2 (toa2) CSB-YP010000SXV
CSB-EP010000SXV
CSB-BP010000SXV
CSB-MP010000SXV
CSB-EP010000SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA replication licensing factor mcm7 (mcm7), partial CSB-YP013597SXV
CSB-EP013597SXV
CSB-BP013597SXV
CSB-MP013597SXV
CSB-EP013597SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-tectorin (TECTA), partial CSB-YP023370HU
CSB-EP023370HU
CSB-BP023370HU
CSB-MP023370HU
CSB-EP023370HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori 60S ribosomal protein L5 (RpL5) CSB-YP020297BTT
CSB-EP020297BTT
CSB-BP020297BTT
CSB-MP020297BTT
CSB-EP020297BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Thymidylate synthase (Ts) CSB-YP025393DLU
CSB-EP025393DLU
CSB-BP025393DLU
CSB-MP025393DLU
CSB-EP025393DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 26S proteasome non-ATPase regulatory subunit 14 (rpn-11) CSB-YP018904CXY
CSB-EP018904CXY
CSB-BP018904CXY
CSB-MP018904CXY
CSB-EP018904CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus rubellus 40S ribosomal protein S10 (RPS10) CSB-YP020359LRJ
CSB-EP020359LRJ
CSB-BP020359LRJ
CSB-MP020359LRJ
CSB-EP020359LRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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