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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Colletotrichum gloeosporioides Cell division control protein 42 homolog (CDC42) CSB-YP005008DVE
CSB-EP005008DVE
CSB-BP005008DVE
CSB-MP005008DVE
CSB-EP005008DVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe FACT complex subunit spt16 (spt16), partial CSB-YP022955SXV
CSB-EP022955SXV
CSB-BP022955SXV
CSB-MP022955SXV
CSB-EP022955SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cytoplasmic tRNA 2-thiolation protein 1 (ncs6) CSB-YP002426SXV
CSB-EP002426SXV
CSB-BP002426SXV
CSB-MP002426SXV
CSB-EP002426SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Sorting nexin-3 (snx3) CSB-YP022374SXV
CSB-EP022374SXV
CSB-BP022374SXV
CSB-MP022374SXV
CSB-EP022374SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Ribose-phosphate pyrophosphokinase 3 (prs3) CSB-YP018777SXV
CSB-EP018777SXV
CSB-BP018777SXV
CSB-MP018777SXV
CSB-EP018777SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 3 subunit E (int6), partial CSB-YP007534SXV
CSB-EP007534SXV
CSB-BP007534SXV
CSB-MP007534SXV
CSB-EP007534SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Prohibitin-2 (phb2), partial CSB-YP017886SXV1
CSB-EP017886SXV1
CSB-BP017886SXV1
CSB-MP017886SXV1
CSB-EP017886SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe JmjC domain-containing protein 4 (jmj4) CSB-YP011952SXV
CSB-EP011952SXV
CSB-BP011952SXV
CSB-MP011952SXV
CSB-EP011952SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerases I and III subunit RPAC1 (rpc40) CSB-YP018324SXV
CSB-EP018324SXV
CSB-BP018324SXV
CSB-MP018324SXV
CSB-EP018324SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Condensin complex subunit 1 (cnd1), partial CSB-YP015514SXV
CSB-EP015514SXV
CSB-BP015514SXV
CSB-MP015514SXV
CSB-EP015514SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum 40S ribosomal protein S30 (RPS30) CSB-YP008447EWP
CSB-EP008447EWP
CSB-BP008447EWP
CSB-MP008447EWP
CSB-EP008447EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Actin-related protein 2/3 complex subunit 2 (arcB) CSB-YP002127DKK
CSB-EP002127DKK
CSB-BP002127DKK
CSB-MP002127DKK
CSB-EP002127DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mandarina 60S ribosomal protein L10 (RpL10) CSB-YP020106BTS
CSB-EP020106BTS
CSB-BP020106BTS
CSB-MP020106BTS
CSB-EP020106BTS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum 40S ribosomal protein S3a (MAL3P7.35, PFC1020c) CSB-YP020444EWP
CSB-EP020444EWP
CSB-BP020444EWP
CSB-MP020444EWP
CSB-EP020444EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Glucosamine-6-phosphate isomerase 1 (GPI1) CSB-YP009630GFW
CSB-EP009630GFW
CSB-BP009630GFW
CSB-MP009630GFW
CSB-EP009630GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca nemestrina Stanniocalcin-2 (STC2) CSB-YP022822MOX
CSB-EP022822MOX
CSB-BP022822MOX
CSB-MP022822MOX
CSB-EP022822MOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis virginiana Beta-adrenergic receptor kinase 1 (ADRBK1), partial CSB-YP001394DKL
CSB-EP001394DKL
CSB-BP001394DKL
CSB-MP001394DKL
CSB-EP001394DKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachypithecus phayrei C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844TPA1
CSB-EP004844TPA1
CSB-BP004844TPA1
CSB-MP004844TPA1
CSB-EP004844TPA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844EXP1
CSB-EP004844EXP1
CSB-BP004844EXP1
CSB-MP004844EXP1
CSB-EP004844EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Alcohol dehydrogenase 1C (ADH1C) CSB-YP001355ERJ
CSB-EP001355ERJ
CSB-BP001355ERJ
CSB-MP001355ERJ
CSB-EP001355ERJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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