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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glycine max DNA polymerase delta catalytic subunit (POLD1), partial CSB-YP018303GGV
CSB-EP018303GGV
CSB-BP018303GGV
CSB-MP018303GGV
CSB-EP018303GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paulownia kawakamii Superoxide dismutase [Cu-Zn] (SODCC) CSB-YP022397ESM
CSB-EP022397ESM
CSB-BP022397ESM
CSB-MP022397ESM
CSB-EP022397ESM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Single-stranded DNA-binding protein (ssb) CSB-YP022706BUD
CSB-EP022706BUD
CSB-BP022706BUD
CSB-MP022706BUD
CSB-EP022706BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ATP synthase subunit a (atpB), partial CSB-YP015070BXE1
CSB-EP015070BXE1
CSB-BP015070BXE1
CSB-MP015070BXE1
CSB-EP015070BXE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine desulfurase (iscS) CSB-YP015769ROT
CSB-EP015769ROT
CSB-BP015769ROT
CSB-MP015769ROT
CSB-EP015769ROT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zona pellucida sperm-binding protein 2 (Zp2), partial CSB-YP027120RA1
CSB-EP027120RA1
CSB-BP027120RA1
CSB-MP027120RA1
CSB-EP027120RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Membrane-bound transcription factor site-2 protease (MBTPS2), partial CSB-YP013557DXU
CSB-EP013557DXU
CSB-BP013557DXU
CSB-MP013557DXU
CSB-EP013557DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Interferon gamma (IFNG) CSB-YP011050DXJ
CSB-EP011050DXJ
CSB-BP011050DXJ
CSB-MP011050DXJ
CSB-EP011050DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Surfeit locus protein 1 (surf1), partial CSB-YP022963TKY1
CSB-EP022963TKY1
CSB-BP022963TKY1
CSB-MP022963TKY1
CSB-EP022963TKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Interferon gamma (IFNG) CSB-YP011050MQV
CSB-EP011050MQV
CSB-BP011050MQV
CSB-MP011050MQV
CSB-EP011050MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Glutamine synthetase (glnA) CSB-YP009553FHX
CSB-EP009553FHX
CSB-BP009553FHX
CSB-MP009553FHX
CSB-EP009553FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Diphthine synthase (dphB) CSB-YP007133FHX
CSB-EP007133FHX
CSB-BP007133FHX
CSB-MP007133FHX
CSB-EP007133FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Transcription initiation factor IIB (tfb) CSB-YP010001FHX
CSB-EP010001FHX
CSB-BP010001FHX
CSB-MP010001FHX
CSB-EP010001FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamine synthetase (glnA) CSB-YP009553MSB
CSB-EP009553MSB
CSB-BP009553MSB
CSB-MP009553MSB
CSB-EP009553MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Geranylgeranyl pyrophosphate synthase (spo9) CSB-YP009393SXV
CSB-EP009393SXV
CSB-BP009393SXV
CSB-MP009393SXV
CSB-EP009393SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exosome complex component csl4 (csl4) CSB-YP007888SXV
CSB-EP007888SXV
CSB-BP007888SXV
CSB-MP007888SXV
CSB-EP007888SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 60S ribosomal protein L5 (rpl-5) CSB-YP020297NHA
CSB-EP020297NHA
CSB-BP020297NHA
CSB-MP020297NHA
CSB-EP020297NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis E3 ubiquitin-protein ligase UBR1 (UBR1), partial CSB-YP025516KBK
CSB-EP025516KBK
CSB-BP025516KBK
CSB-MP025516KBK
CSB-EP025516KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Synaptobrevin homolog ykt6 (ykt6) CSB-YP026265SXV
CSB-EP026265SXV
CSB-BP026265SXV
CSB-MP026265SXV
CSB-EP026265SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cullin-1 (culA), partial CSB-YP006218DKK
CSB-EP006218DKK
CSB-BP006218DKK
CSB-MP006218DKK
CSB-EP006218DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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