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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. ATP-dependent zinc metalloprotease FtsH 4 (ftsH4), partial CSB-YP300372SSQ1
CSB-EP300372SSQ1
CSB-BP300372SSQ1
CSB-MP300372SSQ1
CSB-EP300372SSQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Nitrate transport protein NrtA (nrtA) CSB-YP300373SSQ
CSB-EP300373SSQ
CSB-BP300373SSQ
CSB-MP300373SSQ
CSB-EP300373SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Universal stress protein Slr1230 (slr1230) CSB-YP300374SSQ
CSB-EP300374SSQ
CSB-BP300374SSQ
CSB-MP300374SSQ
CSB-EP300374SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Serine/threonine-protein kinase-like protein E (spkE), partial CSB-YP300375SSQ
CSB-EP300375SSQ
CSB-BP300375SSQ
CSB-MP300375SSQ
CSB-EP300375SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Pyruvate kinase 2 (pyk2), partial CSB-YP300376SSQ
CSB-EP300376SSQ
CSB-BP300376SSQ
CSB-MP300376SSQ
CSB-EP300376SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr0889 (slr0889) CSB-YP300377SSQ
CSB-EP300377SSQ
CSB-BP300377SSQ
CSB-MP300377SSQ
CSB-EP300377SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein sll1783 (sll1783) CSB-YP300378SSQ
CSB-EP300378SSQ
CSB-BP300378SSQ
CSB-MP300378SSQ
CSB-EP300378SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. D-alanine--D-alanine ligase (ddl) CSB-YP300379SSQ
CSB-EP300379SSQ
CSB-BP300379SSQ
CSB-MP300379SSQ
CSB-EP300379SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phosphoglucosamine mutase (glmM) CSB-YP300380SSQ
CSB-EP300380SSQ
CSB-BP300380SSQ
CSB-MP300380SSQ
CSB-EP300380SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 7-carboxy-7-deazaguanine synthase (queE) CSB-YP300381SSQ
CSB-EP300381SSQ
CSB-BP300381SSQ
CSB-MP300381SSQ
CSB-EP300381SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative OxPP cycle protein opcA (opcA) CSB-YP300382SSQ
CSB-EP300382SSQ
CSB-BP300382SSQ
CSB-MP300382SSQ
CSB-EP300382SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP300383SSQ
CSB-EP300383SSQ
CSB-BP300383SSQ
CSB-MP300383SSQ
CSB-EP300383SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr1261 (slr1261), partial CSB-YP300384SSQ1
CSB-EP300384SSQ1
CSB-BP300384SSQ1
CSB-MP300384SSQ1
CSB-EP300384SSQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cyanophycin synthetase (cphA), partial CSB-YP300385SSQ
CSB-EP300385SSQ
CSB-BP300385SSQ
CSB-MP300385SSQ
CSB-EP300385SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP300386SSQ
CSB-EP300386SSQ
CSB-BP300386SSQ
CSB-MP300386SSQ
CSB-EP300386SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative esterase slr0264 (slr0264) CSB-YP300387SSQ
CSB-EP300387SSQ
CSB-BP300387SSQ
CSB-MP300387SSQ
CSB-EP300387SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phycobilisome degradation protein nblA homolog 1 (ssl0452) CSB-YP300388SSQ
CSB-EP300388SSQ
CSB-BP300388SSQ
CSB-MP300388SSQ
CSB-EP300388SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phosphate acyltransferase (plsX) CSB-YP300389SSQ
CSB-EP300389SSQ
CSB-BP300389SSQ
CSB-MP300389SSQ
CSB-EP300389SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr1322 (slr1322) CSB-YP300390SSQ
CSB-EP300390SSQ
CSB-BP300390SSQ
CSB-MP300390SSQ
CSB-EP300390SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP300391SSQ
CSB-EP300391SSQ
CSB-BP300391SSQ
CSB-MP300391SSQ
CSB-EP300391SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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