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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Large-conductance mechanosensitive channel (mscL), partial CSB-YP302405SUL
CSB-EP302405SUL
CSB-BP302405SUL
CSB-MP302405SUL
CSB-EP302405SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP302406FLB
CSB-EP302406FLB
CSB-BP302406FLB
CSB-MP302406FLB
CSB-EP302406FLB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine polyomavirus Small t antigen CSB-YP302407MKH
CSB-EP302407MKH
CSB-BP302407MKH
CSB-MP302407MKH
CSB-EP302407MKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP302408FKZ
CSB-EP302408FKZ
CSB-BP302408FKZ
CSB-MP302408FKZ
CSB-EP302408FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP302409FKZ
CSB-EP302409FKZ
CSB-BP302409FKZ
CSB-MP302409FKZ
CSB-EP302409FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera elata subsp. hookeri Protein psbN (psbN), partial CSB-YP302410OAS
CSB-EP302410OAS
CSB-BP302410OAS
CSB-MP302410OAS
CSB-EP302410OAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP302411SKX
CSB-EP302411SKX
CSB-BP302411SKX
CSB-MP302411SKX
CSB-EP302411SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Gametocyte surface protein P230 (PFS230), partial CSB-YP302412EWP
CSB-BP302412EWP
CSB-MP302412EWP
CSB-EP302412EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP302413ICZ
CSB-EP302413ICZ
CSB-BP302413ICZ
CSB-MP302413ICZ
CSB-EP302413ICZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S2 (rpsB) CSB-YP302414SMT
CSB-EP302414SMT
CSB-BP302414SMT
CSB-MP302414SMT
CSB-EP302414SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv2232/MT2292 (Rv2232, MT2292) CSB-YP302415MVZ
CSB-EP302415MVZ
CSB-BP302415MVZ
CSB-MP302415MVZ
CSB-EP302415MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L25 (rplY) CSB-YP302416ENV
CSB-EP302416ENV
CSB-BP302416ENV
CSB-MP302416ENV
CSB-EP302416ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SF6 Terminase small subunit (1) CSB-YP302417BBH
CSB-EP302417BBH
CSB-BP302417BBH
CSB-MP302417BBH
CSB-EP302417BBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage PZA Early protein GP16.8 (16.8) CSB-YP302418BBE
CSB-EP302418BBE
CSB-BP302418BBE
CSB-MP302418BBE
CSB-EP302418BBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera picensis Putative uncharacterized protein ycf15 (ycf15) CSB-YP302419OAV
CSB-EP302419OAV
CSB-BP302419OAV
CSB-MP302419OAV
CSB-EP302419OAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Early E3 13.3 kDa protein CSB-YP302420CCK
CSB-EP302420CCK
CSB-BP302420CCK
CSB-MP302420CCK
CSB-EP302420CCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Hexon-associated protein (PIX) CSB-YP302421CZQ
CSB-EP302421CZQ
CSB-BP302421CZQ
CSB-MP302421CZQ
CSB-EP302421CZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus C serotype 2 Early E3B 14 kDa protein CSB-YP302422HIK
CSB-EP302422HIK
CSB-BP302422HIK
CSB-MP302422HIK
CSB-EP302422HIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus D serotype 9 Fiber protein (PIV) CSB-YP302423HIP
CSB-EP302423HIP
CSB-BP302423HIP
CSB-MP302423HIP
CSB-EP302423HIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Methionine aminopeptidase (map) CSB-YP302424DUB
CSB-EP302424DUB
CSB-BP302424DUB
CSB-MP302424DUB
CSB-EP302424DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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