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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Triticum aestivum ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP302465TQN
CSB-EP302465TQN
CSB-BP302465TQN
CSB-MP302465TQN
CSB-EP302465TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aegilops crassa ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP302466ADJ
CSB-EP302466ADJ
CSB-BP302466ADJ
CSB-MP302466ADJ
CSB-EP302466ADJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--CoA ligase (fadD) CSB-YP302467EGX
CSB-EP302467EGX
CSB-BP302467EGX
CSB-MP302467EGX
CSB-EP302467EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP302468NHE
CSB-EP302468NHE
CSB-BP302468NHE
CSB-MP302468NHE
CSB-EP302468NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus Coat protein CSB-YP302469TFK
CSB-EP302469TFK
CSB-BP302469TFK
CSB-MP302469TFK
CSB-EP302469TFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nepenthes distillatoria Aspartic proteinase nepenthesin-1 CSB-YP302470NCC
CSB-BP302470NCC
CSB-MP302470NCC
CSB-EP302470NCC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken anemia virus Dual specificity protein phosphatase VP2 (VP2) CSB-YP302471CID
CSB-EP302471CID
CSB-BP302471CID
CSB-MP302471CID
CSB-EP302471CID-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striatus conotoxin S11.3 CSB-YP302472DWI
CSB-EP302472DWI
CSB-BP302472DWI
CSB-MP302472DWI
CSB-EP302472DWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP302473SZB
CSB-EP302473SZB
CSB-BP302473SZB
CSB-MP302473SZB
CSB-EP302473SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato mosaic virus Movement protein (MP) CSB-YP302474TGH
CSB-EP302474TGH
CSB-BP302474TGH
CSB-MP302474TGH
CSB-EP302474TGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Ribonucleoside-diphosphate reductase small chain CSB-YP302475HXA
CSB-EP302475HXA
CSB-BP302475HXA
CSB-MP302475HXA
CSB-EP302475HXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein M (psbM), partial CSB-YP302476LQQ
CSB-EP302476LQQ
CSB-BP302476LQQ
CSB-MP302476LQQ
CSB-EP302476LQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M13 Tail virion protein G7P (VII), partial CSB-YP302477ECY
CSB-EP302477ECY
CSB-BP302477ECY
CSB-MP302477ECY
CSB-EP302477ECY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M13 DNA-Binding protein G5P (V) CSB-YP302478ECY
CSB-EP302478ECY
CSB-BP302478ECY
CSB-MP302478ECY
CSB-EP302478ECY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus contorta Photosystem Q (B) protein, partial CSB-YP302479PKG1
CSB-EP302479PKG1
CSB-BP302479PKG1
CSB-MP302479PKG1
CSB-EP302479PKG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactuca sativa Photosystem Q (B) protein, partial CSB-YP302480LNJ1
CSB-EP302480LNJ1
CSB-BP302480LNJ1
CSB-MP302480LNJ1
CSB-EP302480LNJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium rubrum Photosystem Q (B) protein, partial CSB-YP302481DRT1
CSB-EP302481DRT1
CSB-BP302481DRT1
CSB-MP302481DRT1
CSB-EP302481DRT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudotsuga menziesii Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP302482PWM
CSB-EP302482PWM
CSB-BP302482PWM
CSB-MP302482PWM
CSB-EP302482PWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus aureonitens Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP302483LJV
CSB-EP302483LJV
CSB-BP302483LJV
CSB-MP302483LJV
CSB-EP302483LJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus mutabilis Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP302484LKD
CSB-EP302484LKD
CSB-BP302484LKD
CSB-MP302484LKD
CSB-EP302484LKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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