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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Small heat shock protein hspA (hspA) CSB-YP304903BVW
CSB-EP304903BVW
CSB-BP304903BVW
CSB-MP304903BVW
CSB-EP304903BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis MIP18 family protein yitW (yitW) CSB-YP304904BRJ
CSB-EP304904BRJ
CSB-BP304904BRJ
CSB-MP304904BRJ
CSB-EP304904BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywmC (ywmC) CSB-YP304905BRJ
CSB-EP304905BRJ
CSB-BP304905BRJ
CSB-MP304905BRJ
CSB-EP304905BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywmE (ywmE), partial CSB-YP304906BRJ
CSB-EP304906BRJ
CSB-BP304906BRJ
CSB-MP304906BRJ
CSB-EP304906BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable aminopeptidase ybaC (ybaC) CSB-YP304907BRJ
CSB-EP304907BRJ
CSB-BP304907BRJ
CSB-MP304907BRJ
CSB-EP304907BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Penicillin-binding protein 2D (pbpG), partial CSB-YP304908BRJ
CSB-EP304908BRJ
CSB-BP304908BRJ
CSB-MP304908BRJ
CSB-EP304908BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ABC transporter ATP-binding protein AlbC (albC) CSB-YP304909BRJ
CSB-EP304909BRJ
CSB-BP304909BRJ
CSB-MP304909BRJ
CSB-EP304909BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Septum site-determining protein DivIVA (divIVA) CSB-YP304910BRJ
CSB-EP304910BRJ
CSB-BP304910BRJ
CSB-MP304910BRJ
CSB-EP304910BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywnC (ywnC), partial CSB-YP304911BRJ1
CSB-EP304911BRJ1
CSB-BP304911BRJ1
CSB-MP304911BRJ1
CSB-EP304911BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage II sporulation protein Q (spoIIQ), partial CSB-YP304912BRJ1
CSB-EP304912BRJ1
CSB-BP304912BRJ1
CSB-MP304912BRJ1
CSB-EP304912BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative tyrosine-protein kinase YveL (yveL) CSB-YP304913BRJ
CSB-EP304913BRJ
CSB-BP304913BRJ
CSB-MP304913BRJ
CSB-EP304913BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative pyruvyl transferase epsI (epsI) CSB-YP304914BRJ
CSB-EP304914BRJ
CSB-BP304914BRJ
CSB-MP304914BRJ
CSB-EP304914BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvfG (yvfG) CSB-YP304915BRJ
CSB-EP304915BRJ
CSB-BP304915BRJ
CSB-MP304915BRJ
CSB-EP304915BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0421 protein ygaE (ygaE), partial CSB-YP304916BRJ1
CSB-EP304916BRJ1
CSB-BP304916BRJ1
CSB-MP304916BRJ1
CSB-EP304916BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Acetate kinase (ackA) CSB-YP304917DUB
CSB-EP304917DUB
CSB-BP304917DUB
CSB-MP304917DUB
CSB-EP304917DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative colanic acid biosynthesis glycosyl transferase wcaC (wcaC) CSB-YP304918ENV
CSB-EP304918ENV
CSB-BP304918ENV
CSB-MP304918ENV
CSB-EP304918ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative prophage CP4-6 integrase (intF) CSB-YP304919ENV
CSB-EP304919ENV
CSB-BP304919ENV
CSB-MP304919ENV
CSB-EP304919ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans Chaperone protein DnaK (dnaK), partial CSB-YP304920AYK
CSB-EP304920AYK
CSB-BP304920AYK
CSB-MP304920AYK
CSB-EP304920AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP304921HTA
CSB-EP304921HTA
CSB-BP304921HTA
CSB-MP304921HTA
CSB-EP304921HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Glutamate-pyruvate aminotransferase AlaA (alaA) CSB-YP304922HTA
CSB-EP304922HTA
CSB-BP304922HTA
CSB-MP304922HTA
CSB-EP304922HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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