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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Araneus diadematus Adult-specific rigid cuticular protein 11.9 CSB-YP305308ANI
CSB-EP305308ANI
CSB-BP305308ANI
CSB-MP305308ANI
CSB-EP305308ANI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Araneus diadematus Adult-specific rigid cuticular protein 15.7 CSB-YP305309ANI
CSB-EP305309ANI
CSB-BP305309ANI
CSB-MP305309ANI
CSB-EP305309ANI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri Pyruvate synthase subunit porD (porD) CSB-YP305310MSM
CSB-EP305310MSM
CSB-BP305310MSM
CSB-MP305310MSM
CSB-EP305310MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fasciola hepatica Cathepsin L-like cysteine proteinase, partial CSB-YP305311FEI
CSB-EP305311FEI
CSB-BP305311FEI
CSB-MP305311FEI
CSB-EP305311FEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fasciola hepatica Putative cathepsin-like enzyme CSB-YP305312FEI
CSB-EP305312FEI
CSB-BP305312FEI
CSB-MP305312FEI
CSB-EP305312FEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Jaculus orientalis Glyceraldehyde-3-phosphate dehydrogenase, muscle CSB-YP305313JAB
CSB-EP305313JAB
CSB-BP305313JAB
CSB-MP305313JAB
CSB-EP305313JAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-lactamase CSB-YP305314SFJ
CSB-EP305314SFJ
CSB-BP305314SFJ
CSB-MP305314SFJ
CSB-EP305314SFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus nigrocinctus Short neurotoxin 1 CSB-YP305315MGO
CSB-EP305315MGO
CSB-BP305315MGO
CSB-MP305315MGO
CSB-EP305315MGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Narcissus pseudonarcissus Histone H3 CSB-YP305316NFM
CSB-EP305316NFM
CSB-BP305316NFM
CSB-MP305316NFM
CSB-EP305316NFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Allophycocyanin subunit alpha 1 (apcA1) CSB-YP305317NHR
CSB-EP305317NHR
CSB-BP305317NHR
CSB-MP305317NHR
CSB-EP305317NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aminopeptidase S CSB-YP305318SUW
CSB-EP305318SUW
CSB-BP305318SUW
CSB-MP305318SUW
CSB-EP305318SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrogallol hydroxytransferase large subunit (athL), partial CSB-YP305319PEJ
CSB-EP305319PEJ
CSB-BP305319PEJ
CSB-MP305319PEJ
CSB-EP305319PEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterotoxin CSB-YP305320BQJ
CSB-EP305320BQJ
CSB-BP305320BQJ
CSB-MP305320BQJ
CSB-EP305320BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus galloprovincialis Defensin MGD-1 (FH3) CSB-YP305321MWV
CSB-EP305321MWV
CSB-BP305321MWV
CSB-MP305321MWV
CSB-EP305321MWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Phospho-2-dehydro-3-deoxyheptonate aldolase (aro-8) CSB-YP305322NHA
CSB-EP305322NHA
CSB-BP305322NHA
CSB-MP305322NHA
CSB-EP305322NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maleylpyruvate isomerase (mhbI) CSB-YP305323KBG
CSB-EP305323KBG
CSB-BP305323KBG
CSB-MP305323KBG
CSB-EP305323KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodocyclus tenuis Light-harvesting polypeptide B-885 alpha-1 chain, partial CSB-YP305324RDN
CSB-EP305324RDN
CSB-BP305324RDN
CSB-MP305324RDN
CSB-EP305324RDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodocyclus tenuis Light-harvesting polypeptide B-885 beta-2 chain, partial CSB-YP305325RDN
CSB-EP305325RDN
CSB-BP305325RDN
CSB-MP305325RDN
CSB-EP305325RDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Apyrase (RROP1), partial CSB-YP305326FIG
CSB-EP305326FIG
CSB-BP305326FIG
CSB-MP305326FIG
CSB-EP305326FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida 24 kDa outer membrane protein (PM0998) CSB-YP305327ESG
CSB-EP305327ESG
CSB-BP305327ESG
CSB-MP305327ESG
CSB-EP305327ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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