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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP305227FLV
CSB-EP305227FLV
CSB-BP305227FLV
CSB-MP305227FLV
CSB-EP305227FLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized deoxyribonuclease MG009 homolog (MPN_009) CSB-YP305228MLW
CSB-EP305228MLW
CSB-BP305228MLW
CSB-MP305228MLW
CSB-EP305228MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Phosphoglycerate kinase (pgk) CSB-YP305229MLW
CSB-EP305229MLW
CSB-BP305229MLW
CSB-MP305229MLW
CSB-EP305229MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Lon protease (lon), partial CSB-YP305230MLW
CSB-BP305230MLW
CSB-MP305230MLW
CSB-EP305230MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thiosulfate sulfurtransferase YnjE (ynjE) CSB-YP305231ENV
CSB-EP305231ENV
CSB-BP305231ENV
CSB-MP305231ENV
CSB-EP305231ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Probable ferric reductase transmembrane component (CFL1), partial CSB-YP305232CZF1
CSB-EP305232CZF1
CSB-BP305232CZF1
CSB-MP305232CZF1
CSB-EP305232CZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibberella fujikuroi Nitrogen regulatory protein areA (AREA), partial CSB-YP305233GGA
CSB-EP305233GGA
CSB-BP305233GGA
CSB-MP305233GGA
CSB-EP305233GGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Putative rhamnogalacturonase (asd-1), partial CSB-YP305234NHA
CSB-EP305234NHA
CSB-BP305234NHA
CSB-MP305234NHA
CSB-EP305234NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquitin-conjugating enzyme E2 2 (UBC2) CSB-YP305235NBG
CSB-EP305235NBG
CSB-BP305235NBG
CSB-MP305235NBG
CSB-EP305235NBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Pheromone B beta 1 receptor (BBR1), partial CSB-YP305236SXR1
CSB-EP305236SXR1
CSB-BP305236SXR1
CSB-MP305236SXR1
CSB-EP305236SXR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable U3 small nucleolar RNA-associated protein 18 (SPBC29A3.06), partial CSB-YP305237SXV
CSB-EP305237SXV
CSB-BP305237SXV
CSB-MP305237SXV
CSB-EP305237SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized mitochondrial carrier C83.13 (SPBC83.13), partial CSB-YP305238SXV1
CSB-EP305238SXV1
CSB-BP305238SXV1
CSB-MP305238SXV1
CSB-EP305238SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable UTP--glucose-1-phosphate uridylyltransferase (SPCC1322.04), partial CSB-YP305239SXV
CSB-EP305239SXV
CSB-BP305239SXV
CSB-MP305239SXV
CSB-EP305239SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable ketol-acid reductoisomerase, mitochondrial (ilv5), partial CSB-YP305240SXV
CSB-EP305240SXV
CSB-BP305240SXV
CSB-MP305240SXV
CSB-EP305240SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Lysophospholipase 1 (plb1), partial CSB-YP305241SXV
CSB-EP305241SXV
CSB-BP305241SXV
CSB-MP305241SXV
CSB-EP305241SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable lipase C16A3.12c (SPBC16A3.12c), partial CSB-YP305242SXV
CSB-EP305242SXV
CSB-BP305242SXV
CSB-MP305242SXV
CSB-EP305242SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Sulfate adenylyltransferase (sua1) CSB-YP305243SXV
CSB-EP305243SXV
CSB-BP305243SXV
CSB-MP305243SXV
CSB-EP305243SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Serine/threonine-protein phosphatase PP-Z (pzh1), partial CSB-YP305244SXV
CSB-EP305244SXV
CSB-BP305244SXV
CSB-MP305244SXV
CSB-EP305244SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea jecorina Small COPII coat GTPase sar1 (sar1) CSB-YP305245HYE
CSB-EP305245HYE
CSB-BP305245HYE
CSB-MP305245HYE
CSB-EP305245HYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S ribosomal protein L32-A (rpl32a) CSB-YP305246SXV
CSB-EP305246SXV
CSB-BP305246SXV
CSB-MP305246SXV
CSB-EP305246SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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