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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Treponema hyodysenteriae Flagellar filament outer layer protein flaA2 (flaA2) CSB-YP305267TPQ
CSB-EP305267TPQ
CSB-BP305267TPQ
CSB-MP305267TPQ
CSB-EP305267TPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus terrestris Myosin regulatory light chain, striated muscle, 25 kDa isoform CSB-YP305268LJN
CSB-EP305268LJN
CSB-BP305268LJN
CSB-MP305268LJN
CSB-EP305268LJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 48 kDa protein CSB-YP305269BQJ
CSB-EP305269BQJ
CSB-BP305269BQJ
CSB-MP305269BQJ
CSB-EP305269BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Tetrahydromethanopterin S-methyltransferase subunit D (mtrD), partial CSB-YP305270MSQ1
CSB-EP305270MSQ1
CSB-BP305270MSQ1
CSB-MP305270MSQ1
CSB-EP305270MSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Lysozyme C, kidney isozyme CSB-YP305271SH
CSB-EP305271SH
CSB-BP305271SH
CSB-MP305271SH
CSB-EP305271SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis General stress protein 17M (yflT) CSB-YP305272BRJ
CSB-EP305272BRJ
CSB-BP305272BRJ
CSB-MP305272BRJ
CSB-EP305272BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis Metallothionein 10-II CSB-YP305273MWU
CSB-EP305273MWU
CSB-BP305273MWU
CSB-MP305273MWU
CSB-EP305273MWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L36 (rpmJ) CSB-YP305274TNR
CSB-EP305274TNR
CSB-BP305274TNR
CSB-MP305274TNR
CSB-EP305274TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial CSB-YP305275FIG
CSB-EP305275FIG
CSB-BP305275FIG
CSB-MP305275FIG
CSB-EP305275FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Metallothionein-2B CSB-YP305276RB
CSB-EP305276RB
CSB-BP305276RB
CSB-MP305276RB
CSB-EP305276RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn/Fe] (sodA) CSB-YP305277PSH
CSB-EP305277PSH
CSB-BP305277PSH
CSB-MP305277PSH
CSB-EP305277PSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Ferredoxin-6 CSB-YP305278RLC
CSB-EP305278RLC
CSB-BP305278RLC
CSB-MP305278RLC
CSB-EP305278RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP305279TNR
CSB-EP305279TNR
CSB-BP305279TNR
CSB-MP305279TNR
CSB-EP305279TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteriocin curvaticin FS47 CSB-YP305280LAP
CSB-EP305280LAP
CSB-BP305280LAP
CSB-MP305280LAP
CSB-EP305280LAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia kluyveri Killer toxin KT395 CSB-YP305281PJV
CSB-EP305281PJV
CSB-BP305281PJV
CSB-MP305281PJV
CSB-EP305281PJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-dihydroxybenzoate decarboxylase CSB-YP305282DOZ
CSB-EP305282DOZ
CSB-BP305282DOZ
CSB-MP305282DOZ
CSB-EP305282DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa Avenin-3 CSB-YP305283DPO
CSB-EP305283DPO
CSB-BP305283DPO
CSB-MP305283DPO
CSB-EP305283DPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hevea brasiliensis Pseudo-hevein CSB-YP305284HWI
CSB-EP305284HWI
CSB-BP305284HWI
CSB-MP305284HWI
CSB-EP305284HWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus Metallothionein CSB-YP305285ERM
CSB-EP305285ERM
CSB-BP305285ERM
CSB-MP305285ERM
CSB-EP305285ERM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer membrane protein P2 CSB-YP305286HAM
CSB-EP305286HAM
CSB-BP305286HAM
CSB-MP305286HAM
CSB-EP305286HAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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