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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Cation transport regulator-like protein (SPBC31F10.03) CSB-YP310449SXV
CSB-EP310449SXV
CSB-BP310449SXV
CSB-MP310449SXV
CSB-EP310449SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pre-mRNA-splicing factor clf1 (cwf4), partial CSB-YP310450SXV
CSB-EP310450SXV
CSB-BP310450SXV
CSB-MP310450SXV
CSB-EP310450SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Response regulator mcs4 (mcs4), partial CSB-YP310451SXV
CSB-EP310451SXV
CSB-BP310451SXV
CSB-MP310451SXV
CSB-EP310451SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelophylax ridibundus Somatostatin-1 (sst1) CSB-YP310452PEN
CSB-EP310452PEN
CSB-BP310452PEN
CSB-MP310452PEN
CSB-EP310452PEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP310453IMQ
CSB-EP310453IMQ
CSB-BP310453IMQ
CSB-MP310453IMQ
CSB-EP310453IMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 2 Early E4 30 kDa protein CSB-YP310454CCN
CSB-EP310454CCN
CSB-BP310454CCN
CSB-MP310454CCN
CSB-EP310454CCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cowpox virus Kelch repeat protein C2 (C18L), partial CSB-YP310455DXL
CSB-EP310455DXL
CSB-BP310455DXL
CSB-MP310455DXL
CSB-EP310455DXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibbon hepatitis B virus subtype ayw3q Large envelope protein (S), partial CSB-YP310456GCB1
CSB-EP310456GCB1
CSB-BP310456GCB1
CSB-MP310456GCB1
CSB-EP310456GCB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panicum mosaic virus Protein p15 (ORF4) CSB-YP310457PBA
CSB-EP310457PBA
CSB-BP310457PBA
CSB-MP310457PBA
CSB-EP310457PBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP310458RGX
CSB-EP310458RGX
CSB-BP310458RGX
CSB-MP310458RGX
CSB-EP310458RGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pachytene arrest protein SAE3 (SAE3) CSB-YP310459SVG
CSB-EP310459SVG
CSB-BP310459SVG
CSB-MP310459SVG
CSB-EP310459SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 DNA helicase/primase complex-associated protein (UL8), partial CSB-YP310460HJX
CSB-EP310460HJX
CSB-BP310460HJX
CSB-MP310460HJX
CSB-EP310460HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Protein UL20 (UL20), partial CSB-YP310461HJX1
CSB-BP310461HJX1
CSB-MP310461HJX1
CSB-EP310461HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Major DNA-binding protein (DBP), partial CSB-YP310462HJX
CSB-EP310462HJX
CSB-BP310462HJX
CSB-MP310462HJX
CSB-EP310462HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Deneddylase UL36 (UL36), partial CSB-YP310463HJX
CSB-EP310463HJX
CSB-BP310463HJX
CSB-MP310463HJX
CSB-EP310463HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Triplex capsid protein VP19C (ICP32) CSB-YP310464HJX
CSB-EP310464HJX
CSB-BP310464HJX
CSB-MP310464HJX
CSB-EP310464HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tegument protein VP22 (UL49), partial CSB-YP310465HJX(A4)
CSB-BP310465HJX(A4)
CSB-MP310465HJX(A4)
CSB-EP310465HJX(A4)-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Envelope protein US9 (US9), partial CSB-YP310466HJX1
CSB-EP310466HJX1
CSB-BP310466HJX1
CSB-MP310466HJX1
CSB-EP310466HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato mosaic virus Replicase large subunit, partial CSB-YP310467TGH
CSB-EP310467TGH
CSB-BP310467TGH
CSB-MP310467TGH
CSB-EP310467TGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human spumaretrovirus Protein Bet (bet) CSB-YP310468HQF
CSB-EP310468HQF
CSB-BP310468HQF
CSB-MP310468HQF
CSB-EP310468HQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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