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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Probable ribonuclease VapC39 (vapC39) CSB-YP309435MVZ
CSB-EP309435MVZ
CSB-BP309435MVZ
CSB-MP309435MVZ
CSB-EP309435MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized NAD-dependent oxidoreductase Rv0687/MT0715 (Rv0687, MT0715) CSB-YP309436MVZ
CSB-EP309436MVZ
CSB-BP309436MVZ
CSB-MP309436MVZ
CSB-EP309436MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mycofactocin system heme/flavin oxidoreductase MftD (mftD) CSB-YP309437MVZ
CSB-EP309437MVZ
CSB-BP309437MVZ
CSB-MP309437MVZ
CSB-EP309437MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S17 (rpsQ) CSB-YP309438MVZ
CSB-EP309438MVZ
CSB-BP309438MVZ
CSB-MP309438MVZ
CSB-EP309438MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP309439MVZ
CSB-EP309439MVZ
CSB-BP309439MVZ
CSB-MP309439MVZ
CSB-EP309439MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD29 (fadD29), partial CSB-YP309440MVZ
CSB-EP309440MVZ
CSB-BP309440MVZ
CSB-MP309440MVZ
CSB-EP309440MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB13 (vapB13) CSB-YP309441MVZ
CSB-EP309441MVZ
CSB-BP309441MVZ
CSB-MP309441MVZ
CSB-EP309441MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphatase (hisN) CSB-YP309442MVZ
CSB-EP309442MVZ
CSB-BP309442MVZ
CSB-MP309442MVZ
CSB-EP309442MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant O-succinylhomoserine sulfhydrylase (metZ) CSB-YP309443MVZ
CSB-EP309443MVZ
CSB-BP309443MVZ
CSB-MP309443MVZ
CSB-EP309443MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein mmpL9 (mmpL9), partial CSB-YP309444MVZ
CSB-EP309444MVZ
CSB-BP309444MVZ
CSB-MP309444MVZ
CSB-EP309444MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin HigB (higB) CSB-YP309445MVZ
CSB-EP309445MVZ
CSB-BP309445MVZ
CSB-MP309445MVZ
CSB-EP309445MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin MazF5 (mazF5) CSB-YP309446MVZ
CSB-EP309446MVZ
CSB-BP309446MVZ
CSB-MP309446MVZ
CSB-EP309446MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxococcus xanthus Malate synthase (mls), partial CSB-YP309447MXB
CSB-EP309447MXB
CSB-BP309447MXB
CSB-MP309447MXB
CSB-EP309447MXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein nirL (nirL) CSB-YP309449EZX
CSB-EP309449EZX
CSB-BP309449EZX
CSB-MP309449EZX
CSB-EP309449EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Hydroxydechloroatrazine ethylaminohydrolase (atzB) CSB-YP309450FGF
CSB-EP309450FGF
CSB-BP309450FGF
CSB-MP309450FGF
CSB-EP309450FGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP309451EWY
CSB-EP309451EWY
CSB-BP309451EWY
CSB-MP309451EWY
CSB-EP309451EWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorophyll a/b light-harvesting protein pcbA (pcbA), partial CSB-YP309452PSD1
CSB-EP309452PSD1
CSB-BP309452PSD1
CSB-MP309452PSD1
CSB-EP309452PSD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Integration host factor subunit alpha (ihfA) CSB-YP309454ESE
CSB-EP309454ESE
CSB-BP309454ESE
CSB-MP309454ESE
CSB-EP309454ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP309455SMF
CSB-EP309455SMF
CSB-BP309455SMF
CSB-MP309455SMF
CSB-EP309455SMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR) CSB-YP309456FOA
CSB-EP309456FOA
CSB-BP309456FOA
CSB-MP309456FOA
CSB-EP309456FOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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