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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Ribose-phosphate pyrophosphokinase 4 (prs4) CSB-YP309314SXV
CSB-EP309314SXV
CSB-BP309314SXV
CSB-MP309314SXV
CSB-EP309314SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C3D6.13c (SPBC3D6.13c), partial CSB-YP309315SXV1
CSB-EP309315SXV1
CSB-BP309315SXV1
CSB-MP309315SXV1
CSB-EP309315SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitosis protein dim1 (dim1) CSB-YP309316SXV
CSB-EP309316SXV
CSB-BP309316SXV
CSB-MP309316SXV
CSB-EP309316SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DnaJ homolog 1, mitochondrial (mdj1) CSB-YP309317SXV
CSB-EP309317SXV
CSB-BP309317SXV
CSB-MP309317SXV
CSB-EP309317SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Woronin body major protein (hex-1) CSB-YP309318NHA
CSB-EP309318NHA
CSB-BP309318NHA
CSB-MP309318NHA
CSB-EP309318NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR417C (YDR417C), partial CSB-YP309319SVG1
CSB-EP309319SVG1
CSB-BP309319SVG1
CSB-MP309319SVG1
CSB-EP309319SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR509W (YDR509W), partial CSB-YP309320SVG1
CSB-EP309320SVG1
CSB-BP309320SVG1
CSB-MP309320SVG1
CSB-EP309320SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR274C (YDR274C) CSB-YP309321SVG
CSB-EP309321SVG
CSB-BP309321SVG
CSB-MP309321SVG
CSB-EP309321SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized UPF0442 protein C16A10.01 (SPAC16A10.01), partial CSB-YP309322SXV
CSB-EP309322SXV
CSB-BP309322SXV
CSB-MP309322SXV
CSB-EP309322SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative esterase C31F10.02 (SPBC31F10.02) CSB-YP309323SXV
CSB-EP309323SXV
CSB-BP309323SXV
CSB-MP309323SXV
CSB-EP309323SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe MYND-type zinc finger protein C31F10.10c (SPBC31F10.10c), partial CSB-YP309324SXV
CSB-EP309324SXV
CSB-BP309324SXV
CSB-MP309324SXV
CSB-EP309324SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis T-box protein VegT-A (vegt-a) CSB-YP309325XBE
CSB-EP309325XBE
CSB-BP309325XBE
CSB-MP309325XBE
CSB-EP309325XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African horse sickness virus 6 Non-structural protein NS1 (S5), partial CSB-YP309326AYC
CSB-EP309326AYC
CSB-BP309326AYC
CSB-MP309326AYC
CSB-EP309326AYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cowpox virus Probable host range protein 2 (C4L) CSB-YP309327DXL
CSB-EP309327DXL
CSB-BP309327DXL
CSB-MP309327DXL
CSB-EP309327DXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP309328RGB
CSB-EP309328RGB
CSB-BP309328RGB
CSB-MP309328RGB
CSB-EP309328RGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus OPEP-2 protein (OPEP-2) CSB-YP309329OEU
CSB-EP309329OEU
CSB-BP309329OEU
CSB-MP309329OEU
CSB-EP309329OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus cytomegalovirus Envelope glycoprotein B (gB), partial CSB-YP309330RKC
CSB-BP309330RKC
CSB-MP309330RKC
CSB-EP309330RKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sunn-hemp mosaic virus Replicase large subunit, partial CSB-YP309331SRO
CSB-EP309331SRO
CSB-BP309331SRO
CSB-MP309331SRO
CSB-EP309331SRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Replication origin-binding protein (UL9), partial CSB-YP309332HJX
CSB-EP309332HJX
CSB-BP309332HJX
CSB-MP309332HJX
CSB-EP309332HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Tripartite terminase subunit UL15 homolog (UL15), partial CSB-YP309333HJX
CSB-EP309333HJX
CSB-BP309333HJX
CSB-MP309333HJX
CSB-EP309333HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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