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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Gibberellin 20 oxidase 1 (20ox1) CSB-YP310529OFG
CSB-EP310529OFG
CSB-BP310529OFG
CSB-MP310529OFG
CSB-EP310529OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor 14 (ERF14) CSB-YP310530DOA
CSB-EP310530DOA
CSB-BP310530DOA
CSB-MP310530DOA
CSB-EP310530DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Phospholipase D alpha 2 (PLD2), partial CSB-YP310531OFG
CSB-EP310531OFG
CSB-BP310531OFG
CSB-MP310531OFG
CSB-EP310531OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP310532GCG
CSB-EP310532GCG
CSB-BP310532GCG
CSB-MP310532GCG
CSB-EP310532GCG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Cobalt-zinc-cadmium resistance protein CzcN (czcN), partial CSB-YP310533RJG1
CSB-EP310533RJG1
CSB-BP310533RJG1
CSB-MP310533RJG1
CSB-EP310533RJG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes sp. Cation efflux system protein CzcA (czcA), partial CSB-YP310534AGQ
CSB-EP310534AGQ
CSB-BP310534AGQ
CSB-MP310534AGQ
CSB-EP310534AGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Protein ocs (ocs) CSB-YP310535AYT
CSB-EP310535AYT
CSB-BP310535AYT
CSB-MP310535AYT
CSB-EP310535AYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-layer protein EA1 (eag), partial CSB-YP310536BQE
CSB-EP310536BQE
CSB-BP310536BQE
CSB-MP310536BQE
CSB-EP310536BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L22 (rplV) CSB-YP310537BUD
CSB-EP310537BUD
CSB-BP310537BUD
CSB-MP310537BUD
CSB-EP310537BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus Dipeptide transport system permease protein dppB (dppB), partial CSB-YP310538BRB1
CSB-EP310538BRB1
CSB-BP310538BRB1
CSB-MP310538BRB1
CSB-EP310538BRB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (nodM), partial CSB-YP310539BVW
CSB-EP310539BVW
CSB-BP310539BVW
CSB-MP310539BVW
CSB-EP310539BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP310540DWY
CSB-EP310540DWY
CSB-BP310540DWY
CSB-MP310540DWY
CSB-EP310540DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-binding/permease protein CydD (cydD), partial CSB-YP310541BRJ1
CSB-EP310541BRJ1
CSB-BP310541BRJ1
CSB-MP310541BRJ1
CSB-EP310541BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YxlF (yxlF) CSB-YP310542BRJ
CSB-EP310542BRJ
CSB-BP310542BRJ
CSB-MP310542BRJ
CSB-EP310542BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized symporter ycgO (ycgO), partial CSB-YP310543BRJ
CSB-EP310543BRJ
CSB-BP310543BRJ
CSB-MP310543BRJ
CSB-EP310543BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0718 protein ycgR (ycgR), partial CSB-YP310544BRJ
CSB-EP310544BRJ
CSB-BP310544BRJ
CSB-MP310544BRJ
CSB-EP310544BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yclJ (yclJ) CSB-YP310545BRJ
CSB-EP310545BRJ
CSB-BP310545BRJ
CSB-MP310545BRJ
CSB-EP310545BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ycnI (ycnI), partial CSB-YP310546BRJ
CSB-EP310546BRJ
CSB-BP310546BRJ
CSB-MP310546BRJ
CSB-EP310546BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP310547GFM1
CSB-EP310547GFM1
CSB-BP310547GFM1
CSB-MP310547GFM1
CSB-EP310547GFM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Plipastatin synthase subunit D (ppsD), partial CSB-YP310548BRJ
CSB-EP310548BRJ
CSB-BP310548BRJ
CSB-MP310548BRJ
CSB-EP310548BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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