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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Agaricus bisporus Cellulose-growth-specific protein (cel1) CSB-YP309519AYJ
CSB-EP309519AYJ
CSB-BP309519AYJ
CSB-MP309519AYJ
CSB-EP309519AYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 DNA helicase/primase complex protein (7) CSB-YP309520EFR
CSB-EP309520EFR
CSB-BP309520EFR
CSB-MP309520EFR
CSB-EP309520EFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Norsolorinic acid reductase (norA) CSB-YP309521AVD
CSB-EP309521AVD
CSB-BP309521AVD
CSB-MP309521AVD
CSB-EP309521AVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 (tpsA), partial CSB-YP309522DOZ
CSB-EP309522DOZ
CSB-BP309522DOZ
CSB-MP309522DOZ
CSB-EP309522DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Protein kinase ORF74 (ORF74), partial CSB-YP309523IAD
CSB-EP309523IAD
CSB-BP309523IAD
CSB-MP309523IAD
CSB-EP309523IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF18 (ORF18) CSB-YP309524IAD
CSB-EP309524IAD
CSB-BP309524IAD
CSB-MP309524IAD
CSB-EP309524IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF24 (ORF24) CSB-YP309525IAD
CSB-EP309525IAD
CSB-BP309525IAD
CSB-MP309525IAD
CSB-EP309525IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Subtilisin-like protease (ORF47) CSB-YP309526IAD
CSB-EP309526IAD
CSB-BP309526IAD
CSB-MP309526IAD
CSB-EP309526IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF77 (ORF77) CSB-YP309527IAD
CSB-EP309527IAD
CSB-BP309527IAD
CSB-MP309527IAD
CSB-EP309527IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF66 (ORF66) CSB-YP309528IAD
CSB-EP309528IAD
CSB-BP309528IAD
CSB-MP309528IAD
CSB-EP309528IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF54 (ORF54), partial CSB-YP309529IAD
CSB-EP309529IAD
CSB-BP309529IAD
CSB-MP309529IAD
CSB-EP309529IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Laminin subunit alpha (LanA), partial CSB-YP309530DLU
CSB-EP309530DLU
CSB-BP309530DLU
CSB-MP309530DLU
CSB-EP309530DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein traI (traI) CSB-YP309531ENL
CSB-EP309531ENL
CSB-BP309531ENL
CSB-MP309531ENL
CSB-EP309531ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans pH-response transcription factor pacC/RIM101 (pacC), partial CSB-YP309532EKF
CSB-EP309532EKF
CSB-BP309532EKF
CSB-MP309532EKF
CSB-EP309532EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 (tpsB) CSB-YP309533DOZ
CSB-EP309533DOZ
CSB-BP309533DOZ
CSB-MP309533DOZ
CSB-EP309533DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP309534CTW
CSB-EP309534CTW
CSB-BP309534CTW
CSB-MP309534CTW
CSB-EP309534CTW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium N-hydroxyarylamine O-acetyltransferase (nhoA) CSB-YP309535SXB
CSB-EP309535SXB
CSB-BP309535SXB
CSB-MP309535SXB
CSB-EP309535SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epizootic hemorrhagic disease virus 1 Core protein VP7 (Segment-7) CSB-YP309536EEW
CSB-EP309536EEW
CSB-BP309536EEW
CSB-MP309536EEW
CSB-EP309536EEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Cutinase (cutA) CSB-YP309537BVC
CSB-EP309537BVC
CSB-BP309537BVC
CSB-MP309537BVC
CSB-EP309537BVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eurycotis floridana Hypertrehalosaemic factor CSB-YP309538EIO
CSB-EP309538EIO
CSB-BP309538EIO
CSB-MP309538EIO
CSB-EP309538EIO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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