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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Putative E3 ubiquitin-protein ligase SINAT1 (SINAT1) CSB-YP310126DOA
CSB-EP310126DOA
CSB-BP310126DOA
CSB-MP310126DOA
CSB-EP310126DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum 14-3-3-like protein 16R CSB-YP310127FIG
CSB-EP310127FIG
CSB-BP310127FIG
CSB-MP310127FIG
CSB-EP310127FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Malate dehydrogenase, cytoplasmic 1 (At1g04410) CSB-YP310128DOA
CSB-EP310128DOA
CSB-BP310128DOA
CSB-MP310128DOA
CSB-EP310128DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene response sensor 2 (ERS2), partial CSB-YP310129DOA1
CSB-EP310129DOA1
CSB-BP310129DOA1
CSB-MP310129DOA1
CSB-EP310129DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-isopropylmalate dehydrogenase 2, chloroplastic (IMDH2) CSB-YP310130DOA
CSB-EP310130DOA
CSB-BP310130DOA
CSB-MP310130DOA
CSB-EP310130DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP310131PTD
CSB-EP310131PTD
CSB-BP310131PTD
CSB-MP310131PTD
CSB-EP310131PTD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brownea coccinea Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP310132BMM
CSB-EP310132BMM
CSB-BP310132BMM
CSB-MP310132BMM
CSB-EP310132BMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00170/AtMg00620 (AtMg00170), partial CSB-YP310133DOA1
CSB-EP310133DOA1
CSB-BP310133DOA1
CSB-MP310133DOA1
CSB-EP310133DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 3 member 3 (GER3) CSB-YP310134DOA
CSB-EP310134DOA
CSB-BP310134DOA
CSB-MP310134DOA
CSB-EP310134DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ruegeria gelatinovora Modification methylase AgeI (ageIM) CSB-YP310135RHW
CSB-EP310135RHW
CSB-BP310135RHW
CSB-MP310135RHW
CSB-EP310135RHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric reductase (merA), partial CSB-YP310136AGP
CSB-EP310136AGP
CSB-BP310136AGP
CSB-MP310136AGP
CSB-EP310136AGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycosyltransferase alg8 (alg8), partial CSB-YP310137DPX1
CSB-EP310137DPX1
CSB-BP310137DPX1
CSB-MP310137DPX1
CSB-EP310137DPX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L3 (rplC) CSB-YP310138BUD
CSB-EP310138BUD
CSB-BP310138BUD
CSB-MP310138BUD
CSB-EP310138BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP310139BRB
CSB-EP310139BRB
CSB-BP310139BRB
CSB-MP310139BRB
CSB-EP310139BRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis NAD-specific glutamate dehydrogenase (gdhB) CSB-YP310140BDQ
CSB-EP310140BDQ
CSB-BP310140BDQ
CSB-MP310140BDQ
CSB-EP310140BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Anthranilate phosphoribosyltransferase (trpD) CSB-YP310141BVW
CSB-EP310141BVW
CSB-BP310141BVW
CSB-MP310141BVW
CSB-EP310141BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Cell division protein FtsQ (ftsQ), partial CSB-YP310142DWY
CSB-EP310142DWY
CSB-BP310142DWY
CSB-MP310142DWY
CSB-EP310142DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yxkC (yxkC) CSB-YP310143BRJ
CSB-EP310143BRJ
CSB-BP310143BRJ
CSB-MP310143BRJ
CSB-EP310143BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Citrate/malate transporter (cimH), partial CSB-YP310144BRJ
CSB-EP310144BRJ
CSB-BP310144BRJ
CSB-MP310144BRJ
CSB-EP310144BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ADP-dependent (S)-NAD (P)H-hydrate dehydratase CSB-YP310145BRJ
CSB-EP310145BRJ
CSB-BP310145BRJ
CSB-MP310145BRJ
CSB-EP310145BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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