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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Non-canonical purine NTP pyrophosphatase (ysnA) CSB-YP311337BRJ
CSB-EP311337BRJ
CSB-BP311337BRJ
CSB-MP311337BRJ
CSB-EP311337BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis TPR repeat-containing protein ysoA (ysoA) CSB-YP311338BRJ
CSB-EP311338BRJ
CSB-BP311338BRJ
CSB-MP311338BRJ
CSB-EP311338BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum DNA gyrase subunit A (gyrA), partial CSB-YP311339DUB
CSB-EP311339DUB
CSB-BP311339DUB
CSB-MP311339DUB
CSB-EP311339DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 3-isopropylmalate dehydrogenase (leuB) CSB-YP311340DWY
CSB-EP311340DWY
CSB-BP311340DWY
CSB-MP311340DWY
CSB-EP311340DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit gamma (ureA) CSB-YP311341CMB
CSB-EP311341CMB
CSB-BP311341CMB
CSB-MP311341CMB
CSB-EP311341CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 60 kDa chaperonin (groL), partial CSB-YP311342FCO
CSB-EP311342FCO
CSB-BP311342FCO
CSB-MP311342FCO
CSB-EP311342FCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula vallismortis Cruxrhodopsin-3 (cop3), partial CSB-YP311343HBE1
CSB-EP311343HBE1
CSB-BP311343HBE1
CSB-MP311343HBE1
CSB-EP311343HBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Oligoendopeptidase F, chromosomal (pepF2), partial CSB-YP311344LNF
CSB-EP311344LNF
CSB-BP311344LNF
CSB-MP311344LNF
CSB-EP311344LNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morganella morganii Beta-lactamase (ampC) CSB-YP311345MII
CSB-EP311345MII
CSB-BP311345MII
CSB-MP311345MII
CSB-EP311345MII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine racemase (alr) CSB-YP311346MVW
CSB-EP311346MVW
CSB-BP311346MVW
CSB-MP311346MVW
CSB-EP311346MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyketide synthase-like Pks10 (pks10) CSB-YP311347MVZ
CSB-EP311347MVZ
CSB-BP311347MVZ
CSB-MP311347MVZ
CSB-EP311347MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mycofactocin radical SAM maturase MftC (mftC) CSB-YP311348MVZ
CSB-EP311348MVZ
CSB-BP311348MVZ
CSB-MP311348MVZ
CSB-EP311348MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP311349MVZ
CSB-EP311349MVZ
CSB-BP311349MVZ
CSB-MP311349MVZ
CSB-EP311349MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbon starvation protein A homolog (cstA), partial CSB-YP311350MVZ
CSB-EP311350MVZ
CSB-BP311350MVZ
CSB-MP311350MVZ
CSB-EP311350MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP311351MVZ
CSB-EP311351MVZ
CSB-BP311351MVZ
CSB-MP311351MVZ
CSB-EP311351MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative oxidoreductase Rv1856c/MT1904 (Rv1856c, MT1904) CSB-YP311352MVZ
CSB-EP311352MVZ
CSB-BP311352MVZ
CSB-MP311352MVZ
CSB-EP311352MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Redox sensor histidine kinase response regulator devS (devS), partial CSB-YP311353MVZ
CSB-EP311353MVZ
CSB-BP311353MVZ
CSB-MP311353MVZ
CSB-EP311353MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ESAT-6-like protein 7 (Rv2347c, MT2412) CSB-YP311354MVZ
CSB-EP311354MVZ
CSB-BP311354MVZ
CSB-MP311354MVZ
CSB-EP311354MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxin ParE1 (parE1) CSB-YP311355MVZ
CSB-EP311355MVZ
CSB-BP311355MVZ
CSB-MP311355MVZ
CSB-EP311355MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin MazE5 (mazE5) CSB-YP311356MVZ
CSB-EP311356MVZ
CSB-BP311356MVZ
CSB-MP311356MVZ
CSB-EP311356MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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