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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Protein translocase subunit SecA (secA), partial CSB-YP309457SUW
CSB-EP309457SUW
CSB-BP309457SUW
CSB-MP309457SUW
CSB-EP309457SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP309458FMR
CSB-EP309458FMR
CSB-BP309458FMR
CSB-MP309458FMR
CSB-EP309458FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP309459SPJ
CSB-EP309459SPJ
CSB-BP309459SPJ
CSB-MP309459SPJ
CSB-EP309459SPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Phosphoglycolate phosphatase 2 (SSO2157) CSB-YP309460FPM
CSB-EP309460FPM
CSB-BP309460FPM
CSB-MP309460FPM
CSB-EP309460FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP309461FPM
CSB-EP309461FPM
CSB-BP309461FPM
CSB-MP309461FPM
CSB-EP309461FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus 50S ribosomal protein L13P (rpl13p) CSB-YP309462FPM
CSB-EP309462FPM
CSB-BP309462FPM
CSB-MP309462FPM
CSB-EP309462FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus 50S ribosomal protein L11P (rpl11p) CSB-YP309463FPM
CSB-EP309463FPM
CSB-BP309463FPM
CSB-MP309463FPM
CSB-EP309463FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative 2-aminoethylphosphonate transport system permease protein phnU (phnU), partial CSB-YP309464SXB1
CSB-EP309464SXB1
CSB-BP309464SXB1
CSB-MP309464SXB1
CSB-EP309464SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP309465TNN
CSB-EP309465TNN
CSB-BP309465TNN
CSB-MP309465TNN
CSB-EP309465TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma acidophilum Tricorn protease (tri), partial CSB-YP309466TND
CSB-EP309466TND
CSB-BP309466TND
CSB-MP309466TND
CSB-EP309466TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Zinc transport system membrane protein troD (troD), partial CSB-YP309467TPR
CSB-EP309467TPR
CSB-BP309467TPR
CSB-MP309467TPR
CSB-EP309467TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator in ibpB-leuC intergenic region CSB-YP309469DPX
CSB-EP309469DPX
CSB-BP309469DPX
CSB-MP309469DPX
CSB-EP309469DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant USG-1 protein homolog (usg) CSB-YP309470DPX
CSB-EP309470DPX
CSB-BP309470DPX
CSB-MP309470DPX
CSB-EP309470DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable doxorubicin resistance ABC transporter permease protein drrC (drrC), partial CSB-YP309471MVZ1
CSB-EP309471MVZ1
CSB-BP309471MVZ1
CSB-MP309471MVZ1
CSB-EP309471MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prephenate dehydratase (pheA) CSB-YP309472MVZ
CSB-EP309472MVZ
CSB-BP309472MVZ
CSB-MP309472MVZ
CSB-EP309472MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), partial CSB-YP309473MVZ
CSB-EP309473MVZ
CSB-BP309473MVZ
CSB-MP309473MVZ
CSB-EP309473MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Cold shock protein 2 (cspL) CSB-YP309474LMS
CSB-EP309474LMS
CSB-BP309474LMS
CSB-MP309474LMS
CSB-EP309474LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-isopropylmalate synthase (leuA), partial CSB-YP309475MVZ
CSB-EP309475MVZ
CSB-BP309475MVZ
CSB-MP309475MVZ
CSB-EP309475MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0122 protein SMU_1061 (ylxM) CSB-YP309476FMR
CSB-EP309476FMR
CSB-BP309476FMR
CSB-MP309476FMR
CSB-EP309476FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate synthase component 1 (trpE), partial CSB-YP309477DOH
CSB-EP309477DOH
CSB-BP309477DOH
CSB-MP309477DOH
CSB-EP309477DOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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