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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP309883SKY
CSB-EP309883SKY
CSB-BP309883SKY
CSB-MP309883SKY
CSB-EP309883SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate acetyltransferase (pta) CSB-YP309884SKY
CSB-EP309884SKY
CSB-BP309884SKY
CSB-MP309884SKY
CSB-EP309884SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Superoxide dismutase [Mn/Fe] 1 (sodA) CSB-YP309885SKY
CSB-EP309885SKY
CSB-BP309885SKY
CSB-MP309885SKY
CSB-EP309885SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit beta (dnaN) CSB-YP309886SKY
CSB-EP309886SKY
CSB-BP309886SKY
CSB-MP309886SKY
CSB-EP309886SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-lactate dehydrogenase 2 (ldhB) CSB-YP309887SKY
CSB-EP309887SKY
CSB-BP309887SKY
CSB-MP309887SKY
CSB-EP309887SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable thiol peroxidase (tpx) CSB-YP309888SKY
CSB-EP309888SKY
CSB-BP309888SKY
CSB-MP309888SKY
CSB-EP309888SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable transglycosylase isaA (isaA) CSB-YP309889SKY
CSB-EP309889SKY
CSB-BP309889SKY
CSB-MP309889SKY
CSB-EP309889SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP309890SKY
CSB-EP309890SKY
CSB-BP309890SKY
CSB-MP309890SKY
CSB-EP309890SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable cysteine desulfurase (csd) CSB-YP309891SKY
CSB-EP309891SKY
CSB-BP309891SKY
CSB-MP309891SKY
CSB-EP309891SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein 1a (ORF1a), partial CSB-YP309892BMD
CSB-EP309892BMD
CSB-BP309892BMD
CSB-MP309892BMD
CSB-EP309892BMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transposon gamma-delta 80.3 kDa protein (tnpX) CSB-YP309893ENV
CSB-EP309893ENV
CSB-BP309893ENV
CSB-MP309893ENV
CSB-EP309893ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian foamy virus type 1 Gag polyprotein (gag), partial CSB-YP309894SGP
CSB-EP309894SGP
CSB-BP309894SGP
CSB-MP309894SGP
CSB-EP309894SGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Protein kinase ORF15 (ORF15) CSB-YP309895IAD
CSB-EP309895IAD
CSB-BP309895IAD
CSB-MP309895IAD
CSB-EP309895IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF65 (ORF65), partial CSB-YP309896IAD
CSB-EP309896IAD
CSB-BP309896IAD
CSB-MP309896IAD
CSB-EP309896IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF21 (ORF21) CSB-YP309897IAD
CSB-EP309897IAD
CSB-BP309897IAD
CSB-MP309897IAD
CSB-EP309897IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF41 (ORF41) CSB-YP309898IAD
CSB-EP309898IAD
CSB-BP309898IAD
CSB-MP309898IAD
CSB-EP309898IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF23 (ORF23) CSB-YP309899IAD
CSB-EP309899IAD
CSB-BP309899IAD
CSB-MP309899IAD
CSB-EP309899IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Putative membrane protein ORF10 (ORF10), partial CSB-YP309900IAD1
CSB-EP309900IAD1
CSB-BP309900IAD1
CSB-MP309900IAD1
CSB-EP309900IAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF38 (ORF38) CSB-YP309901IAD
CSB-EP309901IAD
CSB-BP309901IAD
CSB-MP309901IAD
CSB-EP309901IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF76 (ORF76) CSB-YP309902IAD
CSB-EP309902IAD
CSB-BP309902IAD
CSB-MP309902IAD
CSB-EP309902IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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