Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized tatC-like protein ymf16 (YMF16), partial CSB-YP311297DOA1
CSB-EP311297DOA1
CSB-BP311297DOA1
CSB-MP311297DOA1
CSB-EP311297DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Guanine nucleotide-binding protein subunit beta-like protein CSB-YP311298NHC
CSB-EP311298NHC
CSB-BP311298NHC
CSB-MP311298NHC
CSB-EP311298NHC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Actin-54 CSB-YP311299NHE
CSB-EP311299NHE
CSB-BP311299NHE
CSB-MP311299NHE
CSB-EP311299NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Phospholipase D alpha 1 (PLD1), partial CSB-YP311300NHE
CSB-EP311300NHE
CSB-BP311300NHE
CSB-MP311300NHE
CSB-EP311300NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum E3 ubiquitin-protein ligase COP1 (COP1), partial CSB-YP311301EWE
CSB-EP311301EWE
CSB-BP311301EWE
CSB-MP311301EWE
CSB-EP311301EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Oxygen-evolving enhancer protein 2, chloroplastic (PSBP) CSB-YP311302FIG
CSB-EP311302FIG
CSB-BP311302FIG
CSB-MP311302FIG
CSB-EP311302FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Actin-42 CSB-YP311303FIG
CSB-EP311303FIG
CSB-BP311303FIG
CSB-MP311303FIG
CSB-EP311303FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Syntaxin-22 (SYP22), partial CSB-YP311304DOA
CSB-EP311304DOA
CSB-BP311304DOA
CSB-MP311304DOA
CSB-EP311304DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana EC protein homolog 1 (At2g42000) CSB-YP311305DOA
CSB-EP311305DOA
CSB-BP311305DOA
CSB-MP311305DOA
CSB-EP311305DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Phosphoglucomutase, cytoplasmic 2, partial CSB-YP311306ZAX
CSB-EP311306ZAX
CSB-BP311306ZAX
CSB-MP311306ZAX
CSB-EP311306ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 1B (DREB1B) CSB-YP311307DOA
CSB-EP311307DOA
CSB-BP311307DOA
CSB-MP311307DOA
CSB-EP311307DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vicia faba Leghemoglobin 29 (LB29) CSB-YP311308VFJ
CSB-EP311308VFJ
CSB-BP311308VFJ
CSB-MP311308VFJ
CSB-EP311308VFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP311309ANZ
CSB-EP311309ANZ
CSB-BP311309ANZ
CSB-MP311309ANZ
CSB-EP311309ANZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP311310AQZ
CSB-EP311310AQZ
CSB-BP311310AQZ
CSB-MP311310AQZ
CSB-EP311310AQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Metallothionein-like protein 2A (MT2A) CSB-YP311311OFG
CSB-EP311311OFG
CSB-BP311311OFG
CSB-MP311311OFG
CSB-EP311311OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LSD1 (LSD1) CSB-YP311312DOA
CSB-EP311312DOA
CSB-BP311312DOA
CSB-MP311312DOA
CSB-EP311312DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Strictosidine synthase 1 (SS1) CSB-YP311313DOA
CSB-EP311313DOA
CSB-BP311313DOA
CSB-MP311313DOA
CSB-EP311313DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus pinatubonensis Maleylacetate reductase 2 (tfdFII) CSB-YP311314DZR
CSB-EP311314DZR
CSB-BP311314DZR
CSB-MP311314DZR
CSB-EP311314DZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes xylosoxydans xylosoxydans N-acyl-D-aspartate deacylase CSB-YP311315AGR
CSB-EP311315AGR
CSB-BP311315AGR
CSB-MP311315AGR
CSB-EP311315AGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L23 (rplW) CSB-YP311316BUD
CSB-EP311316BUD
CSB-BP311316BUD
CSB-MP311316BUD
CSB-EP311316BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>