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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saimiriine herpesvirus 2 Uncharacterized gene 27 protein (27) CSB-YP311500SAX
CSB-EP311500SAX
CSB-BP311500SAX
CSB-MP311500SAX
CSB-EP311500SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (METK) CSB-YP311501OCT
CSB-EP311501OCT
CSB-BP311501OCT
CSB-MP311501OCT
CSB-EP311501OCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation factor GUF1 homolog, mitochondrial (Ot10g01840) CSB-YP311502OCT
CSB-EP311502OCT
CSB-BP311502OCT
CSB-MP311502OCT
CSB-EP311502OCT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Uncharacterized gene 23 protein (23) CSB-YP311503SAX
CSB-EP311503SAX
CSB-BP311503SAX
CSB-MP311503SAX
CSB-EP311503SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Probable ATP-dependent helicase 44 (44), partial CSB-YP311504SAX
CSB-EP311504SAX
CSB-BP311504SAX
CSB-MP311504SAX
CSB-EP311504SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Gene 35 protein (35) CSB-YP311505SAX
CSB-EP311505SAX
CSB-BP311505SAX
CSB-MP311505SAX
CSB-EP311505SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Uncharacterized gene 48 protein (48), partial CSB-YP311506SAX
CSB-EP311506SAX
CSB-BP311506SAX
CSB-MP311506SAX
CSB-EP311506SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Cyclin homolog (72) CSB-YP311507SAX
CSB-EP311507SAX
CSB-BP311507SAX
CSB-MP311507SAX
CSB-EP311507SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Probable large tegument protein (64), partial CSB-YP311508SAX
CSB-EP311508SAX
CSB-BP311508SAX
CSB-MP311508SAX
CSB-EP311508SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio gigas Flavodoxin CSB-YP311510DJO
CSB-EP311510DJO
CSB-BP311510DJO
CSB-MP311510DJO
CSB-EP311510DJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae mRNA-capping enzyme subunit alpha (CEG1) CSB-YP311511SVG
CSB-EP311511SVG
CSB-BP311511SVG
CSB-MP311511SVG
CSB-EP311511SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Nitrogen fixation protein nifU (nifU) CSB-YP311512RLE
CSB-EP311512RLE
CSB-BP311512RLE
CSB-MP311512RLE
CSB-EP311512RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum Protein E6 (E6) CSB-YP311513GHB
CSB-EP311513GHB
CSB-BP311513GHB
CSB-MP311513GHB
CSB-EP311513GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow mosaic virus Genome polyprotein 2 (RNA2), partial CSB-YP311514BET
CSB-EP311514BET
CSB-BP311514BET
CSB-MP311514BET
CSB-EP311514BET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus Putative transcription factor MTF1 (MTF1) CSB-YP311515MIW
CSB-EP311515MIW
CSB-BP311515MIW
CSB-MP311515MIW
CSB-EP311515MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation lipoprotein J (yscJ), partial CSB-YP311516YAQ1
CSB-EP311516YAQ1
CSB-BP311516YAQ1
CSB-MP311516YAQ1
CSB-EP311516YAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Protease I (I) CSB-YP311517EDA
CSB-EP311517EDA
CSB-BP311517EDA
CSB-MP311517EDA
CSB-EP311517EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Chalcone synthase 2 (CHS2) CSB-YP311518EWE
CSB-EP311518EWE
CSB-BP311518EWE
CSB-MP311518EWE
CSB-EP311518EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ricinus communis Catalase isozyme 1 (CAT1) CSB-YP311519RMM
CSB-EP311519RMM
CSB-BP311519RMM
CSB-MP311519RMM
CSB-EP311519RMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Clock-controlled protein 8 (ccg-8), partial CSB-YP311520NHA
CSB-EP311520NHA
CSB-BP311520NHA
CSB-MP311520NHA
CSB-EP311520NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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