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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Biopolymer transport protein exbB (exbB), partial CSB-YP309822NGI1
CSB-EP309822NGI1
CSB-BP309822NGI1
CSB-MP309822NGI1
CSB-EP309822NGI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP309823NBM
CSB-EP309823NBM
CSB-BP309823NBM
CSB-MP309823NBM
CSB-EP309823NBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein nirH (nirH) CSB-YP309824EZX
CSB-EP309824EZX
CSB-BP309824EZX
CSB-MP309824EZX
CSB-EP309824EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP309825ESE
CSB-EP309825ESE
CSB-BP309825ESE
CSB-MP309825ESE
CSB-EP309825ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Catalase HPII (katE), partial CSB-YP309826FFZ
CSB-EP309826FFZ
CSB-BP309826FFZ
CSB-MP309826FFZ
CSB-EP309826FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. syringae Protein-export membrane protein SecG (secG), partial CSB-YP309828PWE1
CSB-EP309828PWE1
CSB-BP309828PWE1
CSB-MP309828PWE1
CSB-EP309828PWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides 60 kDa chaperonin 2 (groL2), partial CSB-YP309829RLE
CSB-EP309829RLE
CSB-BP309829RLE
CSB-MP309829RLE
CSB-EP309829RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodovulum sulfidophilum Light-harvesting protein B-800/850 beta chain (pucB), partial CSB-YP309830RMF
CSB-EP309830RMF
CSB-BP309830RMF
CSB-MP309830RMF
CSB-EP309830RMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodovulum sulfidophilum Protein pucC (pucC), partial CSB-YP309831RMF
CSB-EP309831RMF
CSB-BP309831RMF
CSB-MP309831RMF
CSB-EP309831RMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Alpha-acetolactate decarboxylase (aldB) CSB-YP309832LNG
CSB-EP309832LNG
CSB-BP309832LNG
CSB-MP309832LNG
CSB-EP309832LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar hook-basal body complex protein FliE (fliE) CSB-YP309833SGD
CSB-EP309833SGD
CSB-BP309833SGD
CSB-MP309833SGD
CSB-EP309833SGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP309834SUW
CSB-EP309834SUW
CSB-BP309834SUW
CSB-MP309834SUW
CSB-EP309834SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 60 kDa chaperonin (groL), partial CSB-YP309835SLU
CSB-EP309835SLU
CSB-BP309835SLU
CSB-MP309835SLU
CSB-EP309835SLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrolide efflux protein A (mefA), partial CSB-YP309836SMR
CSB-EP309836SMR
CSB-BP309836SMR
CSB-MP309836SMR
CSB-EP309836SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Leucyl aminopeptidase (ape2), partial CSB-YP309837FPM
CSB-EP309837FPM
CSB-BP309837FPM
CSB-MP309837FPM
CSB-EP309837FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus UPF0095 protein SSO0079 (SSO0079) CSB-YP309838FPM
CSB-EP309838FPM
CSB-BP309838FPM
CSB-MP309838FPM
CSB-EP309838FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus 50S ribosomal protein L18e (rpl18e) CSB-YP309839FPM
CSB-EP309839FPM
CSB-BP309839FPM
CSB-MP309839FPM
CSB-EP309839FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative 2-aminoethylphosphonate-binding periplasmic protein (phnS) CSB-YP309840SXB
CSB-EP309840SXB
CSB-BP309840SXB
CSB-MP309840SXB
CSB-EP309840SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma acidophilum Proline iminopeptidase (pip) CSB-YP309841TND
CSB-EP309841TND
CSB-BP309841TND
CSB-MP309841TND
CSB-EP309841TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima Glutamate dehydrogenase (gdhA) CSB-YP309842TNJ
CSB-EP309842TNJ
CSB-BP309842TNJ
CSB-MP309842TNJ
CSB-EP309842TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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