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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Sensor histidine kinase ydfH (ydfH), partial CSB-YP309863BRJ
CSB-EP309863BRJ
CSB-BP309863BRJ
CSB-MP309863BRJ
CSB-EP309863BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore coat protein P (cotP) CSB-YP309864BRJ
CSB-EP309864BRJ
CSB-BP309864BRJ
CSB-MP309864BRJ
CSB-EP309864BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydgG (ydgG) CSB-YP309865BRJ
CSB-EP309865BRJ
CSB-BP309865BRJ
CSB-MP309865BRJ
CSB-EP309865BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Arabinose-proton symporter (araE), partial CSB-YP309866BRJ
CSB-EP309866BRJ
CSB-BP309866BRJ
CSB-MP309866BRJ
CSB-EP309866BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tyrosine-protein kinase YwqD (ywqD) CSB-YP309867BRJ
CSB-EP309867BRJ
CSB-BP309867BRJ
CSB-MP309867BRJ
CSB-EP309867BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0720 protein ywqJ (ywqJ), partial CSB-YP309868BRJ
CSB-EP309868BRJ
CSB-BP309868BRJ
CSB-MP309868BRJ
CSB-EP309868BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywsA (ywsA) CSB-YP309869BRJ
CSB-EP309869BRJ
CSB-BP309869BRJ
CSB-MP309869BRJ
CSB-EP309869BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gamma-DL-glutamyl hydrolase (pgdS) CSB-YP309870BRJ
CSB-EP309870BRJ
CSB-BP309870BRJ
CSB-MP309870BRJ
CSB-EP309870BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri Uncharacterized protein Mbar_A1602 (Mbar_A1602) CSB-YP309871MSM
CSB-EP309871MSM
CSB-BP309871MSM
CSB-MP309871MSM
CSB-EP309871MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional repressor KstR (kstR) CSB-YP309872MVZ
CSB-EP309872MVZ
CSB-BP309872MVZ
CSB-MP309872MVZ
CSB-EP309872MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease VapC5 (vapC5) CSB-YP309873MVZ
CSB-EP309873MVZ
CSB-BP309873MVZ
CSB-MP309873MVZ
CSB-EP309873MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar basal body rod protein FlgB (flgB) CSB-YP309874EYY
CSB-EP309874EYY
CSB-BP309874EYY
CSB-MP309874EYY
CSB-EP309874EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. CR (VI) reductase CSB-YP309875PVM
CSB-EP309875PVM
CSB-BP309875PVM
CSB-MP309875PVM
CSB-EP309875PVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Nitrile hydratase subunit alpha (nthA) CSB-YP309876FFZ
CSB-EP309876FFZ
CSB-BP309876FFZ
CSB-MP309876FFZ
CSB-EP309876FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquinol oxidase subunit 1 (cyaA), partial CSB-YP309877AAX
CSB-EP309877AAX
CSB-BP309877AAX
CSB-MP309877AAX
CSB-EP309877AAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc metalloproteinase nas-28 (nas-28) CSB-YP309878CXY
CSB-EP309878CXY
CSB-BP309878CXY
CSB-MP309878CXY
CSB-EP309878CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Protein SpAN (SPAN), partial CSB-YP309879FOT
CSB-EP309879FOT
CSB-BP309879FOT
CSB-MP309879FOT
CSB-EP309879FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Submaxillary mucin-like protein, partial CSB-YP309880BO
CSB-EP309880BO
CSB-BP309880BO
CSB-MP309880BO
CSB-EP309880BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Unknown protein from spot 2D-000JYC of 2D-PAGE CSB-YP309881SAC
CSB-EP309881SAC
CSB-BP309881SAC
CSB-MP309881SAC
CSB-EP309881SAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP309882SKY
CSB-EP309882SKY
CSB-BP309882SKY
CSB-MP309882SKY
CSB-EP309882SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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