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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human herpesvirus 2 Alpha trans-inducing factor 78 kDa protein (UL46), partial CSB-YP310853HJX
CSB-EP310853HJX
CSB-BP310853HJX
CSB-MP310853HJX
CSB-EP310853HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Transcriptional regulator ICP22 (US1) CSB-YP310854HJX
CSB-EP310854HJX
CSB-BP310854HJX
CSB-MP310854HJX
CSB-EP310854HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Virion protein US10 (US10) CSB-YP310855HJX
CSB-EP310855HJX
CSB-BP310855HJX
CSB-MP310855HJX
CSB-EP310855HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pepper mild mottle virus Replicase large subunit, partial CSB-YP310856PFJ
CSB-EP310856PFJ
CSB-BP310856PFJ
CSB-MP310856PFJ
CSB-EP310856PFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco streak virus Protein 2b (ORF2b) CSB-YP310857TFN
CSB-EP310857TFN
CSB-BP310857TFN
CSB-MP310857TFN
CSB-EP310857TFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 8 type P E3 ubiquitin-protein ligase MIR2 (K5), partial CSB-YP310858HKE
CSB-EP310858HKE
CSB-BP310858HKE
CSB-MP310858HKE
CSB-EP310858HKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DNA topoisomerase 2, mitochondrial (top2mt), partial CSB-YP310859DKK
CSB-EP310859DKK
CSB-BP310859DKK
CSB-MP310859DKK
CSB-EP310859DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase [NAD (+)], glycosomal CSB-YP310860TQV
CSB-EP310860TQV
CSB-BP310860TQV
CSB-MP310860TQV
CSB-EP310860TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fanconi-associated nuclease 1 homolog (fan-1), partial CSB-YP310861CXY
CSB-EP310861CXY
CSB-BP310861CXY
CSB-MP310861CXY
CSB-EP310861CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DDB1- and CUL4-associated factor 11 homolog (wdr-23), partial CSB-YP310862CXY
CSB-EP310862CXY
CSB-BP310862CXY
CSB-MP310862CXY
CSB-EP310862CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant BTB and MATH domain-containing protein 39 (bath-39) CSB-YP310863CXY
CSB-EP310863CXY
CSB-BP310863CXY
CSB-MP310863CXY
CSB-EP310863CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Intermediate filament protein ifa-4 (ifa-4), partial CSB-YP310864CXY
CSB-EP310864CXY
CSB-BP310864CXY
CSB-MP310864CXY
CSB-EP310864CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable phenylalanine-4-hydroxylase 1 (pah-1) CSB-YP310865CXY
CSB-EP310865CXY
CSB-BP310865CXY
CSB-MP310865CXY
CSB-EP310865CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative nudix hydrolase 7 (ndx-7) CSB-YP310866CXY
CSB-EP310866CXY
CSB-BP310866CXY
CSB-MP310866CXY
CSB-EP310866CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase F21F3.1 (F21F3.1) CSB-YP310867CXY
CSB-EP310867CXY
CSB-BP310867CXY
CSB-MP310867CXY
CSB-EP310867CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable V-type proton ATPase subunit G (vha-10) CSB-YP310868CXY
CSB-EP310868CXY
CSB-BP310868CXY
CSB-MP310868CXY
CSB-EP310868CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable phosphoglycerate kinase (pgk-1) CSB-YP310869CXY
CSB-EP310869CXY
CSB-BP310869CXY
CSB-MP310869CXY
CSB-EP310869CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribosome-recycling factor, mitochondrial (T20F5.3), partial CSB-YP310870CXY
CSB-EP310870CXY
CSB-BP310870CXY
CSB-MP310870CXY
CSB-EP310870CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein still life, isoforms C/SIF type 2 (sif), partial CSB-YP310871DLU
CSB-EP310871DLU
CSB-BP310871DLU
CSB-MP310871DLU
CSB-EP310871DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila miranda Larval cuticle protein 1 (Lcp1) CSB-YP310872DLX
CSB-EP310872DLX
CSB-BP310872DLX
CSB-MP310872DLX
CSB-EP310872DLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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