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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S9 (rpsI) CSB-YP311661PUC
CSB-EP311661PUC
CSB-BP311661PUC
CSB-MP311661PUC
CSB-EP311661PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP311662PUC
CSB-EP311662PUC
CSB-BP311662PUC
CSB-MP311662PUC
CSB-EP311662PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Alanine--tRNA ligase (alaS), partial CSB-YP311663PUC
CSB-EP311663PUC
CSB-BP311663PUC
CSB-MP311663PUC
CSB-EP311663PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP311664PUC
CSB-EP311664PUC
CSB-BP311664PUC
CSB-MP311664PUC
CSB-EP311664PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Flagellar hook-basal body complex protein FliE (fliE) CSB-YP311665PUC
CSB-EP311665PUC
CSB-BP311665PUC
CSB-MP311665PUC
CSB-EP311665PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0745 protein PA14_47450 (PA14_47450) CSB-YP311666PUC
CSB-EP311666PUC
CSB-BP311666PUC
CSB-MP311666PUC
CSB-EP311666PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP311667PUC
CSB-EP311667PUC
CSB-BP311667PUC
CSB-MP311667PUC
CSB-EP311667PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA ligase (ligA), partial CSB-YP311668PUC
CSB-EP311668PUC
CSB-BP311668PUC
CSB-MP311668PUC
CSB-EP311668PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Undecaprenyl-diphosphatase (uppP), partial CSB-YP311669PUC1
CSB-EP311669PUC1
CSB-BP311669PUC1
CSB-MP311669PUC1
CSB-EP311669PUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Serine/threonine transporter sstT (sstT), partial CSB-YP311670PUC
CSB-EP311670PUC
CSB-BP311670PUC
CSB-MP311670PUC
CSB-EP311670PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0317 protein PA14_37210 (PA14_37210) CSB-YP311671PUC
CSB-EP311671PUC
CSB-BP311671PUC
CSB-MP311671PUC
CSB-EP311671PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Siroheme synthase (cysG) CSB-YP311672PUC
CSB-EP311672PUC
CSB-BP311672PUC
CSB-MP311672PUC
CSB-EP311672PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP311673PUC
CSB-EP311673PUC
CSB-BP311673PUC
CSB-MP311673PUC
CSB-EP311673PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ecotin (eco) CSB-YP311674PUC
CSB-EP311674PUC
CSB-BP311674PUC
CSB-MP311674PUC
CSB-EP311674PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Molybdenum cofactor guanylyltransferase (mobA) CSB-YP311675PUC
CSB-EP311675PUC
CSB-BP311675PUC
CSB-MP311675PUC
CSB-EP311675PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP311676PUC
CSB-EP311676PUC
CSB-BP311676PUC
CSB-MP311676PUC
CSB-EP311676PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0060 membrane protein PA14_21660 (PA14_21660), partial CSB-YP311677PUC1
CSB-EP311677PUC1
CSB-BP311677PUC1
CSB-MP311677PUC1
CSB-EP311677PUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable D-serine dehydratase (dsdA) CSB-YP311678PUC
CSB-EP311678PUC
CSB-BP311678PUC
CSB-MP311678PUC
CSB-EP311678PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0502 protein PA14_19450 (PA14_19450) CSB-YP311679PUC
CSB-EP311679PUC
CSB-BP311679PUC
CSB-MP311679PUC
CSB-EP311679PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Electron transport complex protein RnfG (rnfG), partial CSB-YP311680PUC1
CSB-EP311680PUC1
CSB-BP311680PUC1
CSB-MP311680PUC1
CSB-EP311680PUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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