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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Uncharacterized protein C4G3.03 (SPCC4G3.03) CSB-YP310832SXV
CSB-EP310832SXV
CSB-BP310832SXV
CSB-MP310832SXV
CSB-EP310832SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe CUE domain-containing protein 4, mitochondrial (SPCC4G3.13c) CSB-YP310833SXV
CSB-EP310833SXV
CSB-BP310833SXV
CSB-MP310833SXV
CSB-EP310833SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C4G3.18 (SPCC4G3.18), partial CSB-YP310834SXV
CSB-EP310834SXV
CSB-BP310834SXV
CSB-MP310834SXV
CSB-EP310834SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydrogenase B (leu2B) CSB-YP310835DOZ
CSB-EP310835DOZ
CSB-BP310835DOZ
CSB-MP310835DOZ
CSB-EP310835DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR455C (YDR455C) CSB-YP310836SVG
CSB-EP310836SVG
CSB-BP310836SVG
CSB-MP310836SVG
CSB-EP310836SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR271C (YDR271C) CSB-YP310837SVG
CSB-EP310837SVG
CSB-BP310837SVG
CSB-MP310837SVG
CSB-EP310837SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Mitochondrial transcription factor 1 (MTF1) CSB-YP310838LMA
CSB-EP310838LMA
CSB-BP310838LMA
CSB-MP310838LMA
CSB-EP310838LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Meiotically up-regulated gene 37 protein (mug37) CSB-YP310839SXV
CSB-EP310839SXV
CSB-BP310839SXV
CSB-MP310839SXV
CSB-EP310839SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein hir1 (hip1), partial CSB-YP310840SXV
CSB-EP310840SXV
CSB-BP310840SXV
CSB-MP310840SXV
CSB-EP310840SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA polymerase subunit delta-2 (cdc1) CSB-YP310841SXV
CSB-EP310841SXV
CSB-BP310841SXV
CSB-MP310841SXV
CSB-EP310841SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acipenser transmontanus Pro-opiomelanocortin (pomc) CSB-YP310842AWZ
CSB-EP310842AWZ
CSB-BP310842AWZ
CSB-MP310842AWZ
CSB-EP310842AWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HERV-K_5q33.3 provirus ancestral Gag polyprotein CSB-YP310844HU
CSB-EP310844HU
CSB-BP310844HU
CSB-MP310844HU
CSB-EP310844HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panicum mosaic virus Capsid protein (ORF3) CSB-YP310845PBA
CSB-EP310845PBA
CSB-BP310845PBA
CSB-MP310845PBA
CSB-EP310845PBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP310846RGR
CSB-EP310846RGR
CSB-BP310846RGR
CSB-MP310846RGR
CSB-EP310846RGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein DDR2 (DDR2) CSB-YP310847SVG
CSB-EP310847SVG
CSB-BP310847SVG
CSB-MP310847SVG
CSB-EP310847SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Cytoplasmic envelopment protein 1 (UL7) CSB-YP310848HJX
CSB-EP310848HJX
CSB-BP310848HJX
CSB-MP310848HJX
CSB-EP310848HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Serine/threonine-protein kinase UL13 (UL13), partial CSB-YP310849HJX
CSB-EP310849HJX
CSB-BP310849HJX
CSB-MP310849HJX
CSB-EP310849HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Major capsid protein (UL19), partial CSB-YP310850HJX
CSB-EP310850HJX
CSB-BP310850HJX
CSB-MP310850HJX
CSB-EP310850HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Protease precursor (UL26), partial CSB-YP310851HJX
CSB-EP310851HJX
CSB-BP310851HJX
CSB-MP310851HJX
CSB-EP310851HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Virion egress protein UL34 (UL34), partial CSB-YP310852HJX1
CSB-EP310852HJX1
CSB-BP310852HJX1
CSB-MP310852HJX1
CSB-EP310852HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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