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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Barley mild mosaic virus Genome polyprotein 2 (RNA2), partial CSB-YP311257BEL
CSB-EP311257BEL
CSB-BP311257BEL
CSB-MP311257BEL
CSB-EP311257BEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barmah forest virus Structural polyprotein, partial CSB-YP311258BEU1
CSB-EP311258BEU1
CSB-BP311258BEU1
CSB-MP311258BEU1
CSB-EP311258BEU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YBR109W-A (YBR109W-A), partial CSB-YP311259SVG1
CSB-EP311259SVG1
CSB-BP311259SVG1
CSB-MP311259SVG1
CSB-EP311259SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutathionylspermidine synthase (GSP), partial CSB-YP311260DXW
CSB-EP311260DXW
CSB-BP311260DXW
CSB-MP311260DXW
CSB-EP311260DXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi 60S ribosomal protein L44 (rpl-44) CSB-YP311261BWV
CSB-EP311261BWV
CSB-BP311261BWV
CSB-MP311261BWV
CSB-EP311261BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative stoned B-like protein (unc-41), partial CSB-YP311262CXY
CSB-EP311262CXY
CSB-BP311262CXY
CSB-MP311262CXY
CSB-EP311262CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc metalloproteinase nas-9 (nas-9), partial CSB-YP311263CXY
CSB-EP311263CXY
CSB-BP311263CXY
CSB-MP311263CXY
CSB-EP311263CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable glutathione S-transferase 6 (gst-6) CSB-YP311264CXY
CSB-EP311264CXY
CSB-BP311264CXY
CSB-MP311264CXY
CSB-EP311264CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis remanei Protein lin-28 (lin-28) CSB-YP311265CXZ
CSB-EP311265CXZ
CSB-BP311265CXZ
CSB-MP311265CXZ
CSB-EP311265CXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster [Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial (Pdk), partial CSB-YP311266DLU
CSB-EP311266DLU
CSB-BP311266DLU
CSB-MP311266DLU
CSB-EP311266DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Translationally-controlled tumor protein homolog (TCTP) CSB-YP311267SXP
CSB-EP311267SXP
CSB-BP311267SXP
CSB-MP311267SXP
CSB-EP311267SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class gamma-46 (srg-46), partial CSB-YP311268CXY1
CSB-EP311268CXY1
CSB-BP311268CXY1
CSB-MP311268CXY1
CSB-EP311268CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble guanylate cyclase gcy-34 (gcy-34), partial CSB-YP311269CXY
CSB-EP311269CXY
CSB-BP311269CXY
CSB-MP311269CXY
CSB-EP311269CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis elegans Dauer larva development regulatory growth factor daf-7 (daf-7) CSB-YP311270CXY
CSB-BP311270CXY
CSB-MP311270CXY
CSB-EP311270CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein stand still (stil) CSB-YP311271DLU
CSB-EP311271DLU
CSB-BP311271DLU
CSB-MP311271DLU
CSB-EP311271DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thinopyrum bessarabicum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP311272TDY
CSB-EP311272TDY
CSB-BP311272TDY
CSB-MP311272TDY
CSB-EP311272TDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00740 (AtMg00740) CSB-YP311273DOA
CSB-EP311273DOA
CSB-BP311273DOA
CSB-MP311273DOA
CSB-EP311273DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01060 (AtMg01060) CSB-YP311274DOA
CSB-EP311274DOA
CSB-BP311274DOA
CSB-MP311274DOA
CSB-EP311274DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP synthase subunit a-2 (ATP6-2), partial CSB-YP311275DOA1
CSB-EP311275DOA1
CSB-BP311275DOA1
CSB-MP311275DOA1
CSB-EP311275DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01240 (AtMg01240) CSB-YP311276DOA
CSB-EP311276DOA
CSB-BP311276DOA
CSB-MP311276DOA
CSB-EP311276DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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