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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Colletotrichum gloeosporioides Protein CAP22 (CAP22) CSB-YP311460DVE
CSB-EP311460DVE
CSB-BP311460DVE
CSB-MP311460DVE
CSB-EP311460DVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coenzyme F420 hydrogenase subunit beta (frhB) CSB-YP311461MSD
CSB-EP311461MSD
CSB-BP311461MSD
CSB-MP311461MSD
CSB-EP311461MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F420-non-reducing hydrogenase vhc subunit G (vhcG) CSB-YP311462MSD
CSB-EP311462MSD
CSB-BP311462MSD
CSB-MP311462MSD
CSB-EP311462MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Helicase SEN1 (SEN1), partial CSB-YP311463SVG
CSB-EP311463SVG
CSB-BP311463SVG
CSB-MP311463SVG
CSB-EP311463SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis B box-binding protein CSB-YP311464XBE
CSB-EP311464XBE
CSB-BP311464XBE
CSB-MP311464XBE
CSB-EP311464XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Small heat shock protein, chloroplastic (HSP21) CSB-YP311465TQN
CSB-EP311465TQN
CSB-BP311465TQN
CSB-MP311465TQN
CSB-EP311465TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP311466DSJ
CSB-EP311466DSJ
CSB-BP311466DSJ
CSB-MP311466DSJ
CSB-EP311466DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cymbidium mosaic virus Movement protein TGBp3 (ORF4), partial CSB-YP311467CVM
CSB-EP311467CVM
CSB-BP311467CVM
CSB-MP311467CVM
CSB-EP311467CVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Botulinum neurotoxin type E, partial CSB-YP311468CLQ
CSB-BP311468CLQ
CSB-MP311468CLQ
CSB-EP311468CLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Type II secretion system protein K (xcpX), partial CSB-YP311469EZX
CSB-EP311469EZX
CSB-BP311469EZX
CSB-MP311469EZX
CSB-EP311469EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Lactococcin-A transport/processing ATP-binding protein LcnC (lcnC), partial CSB-YP311470LNI1
CSB-EP311470LNI1
CSB-BP311470LNI1
CSB-MP311470LNI1
CSB-EP311470LNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helenium virus S 12.6 kDa protein CSB-YP311471HCL
CSB-EP311471HCL
CSB-BP311471HCL
CSB-MP311471HCL
CSB-EP311471HCL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GTP-binding protein GTR1 (GTR1) CSB-YP311472SVG
CSB-EP311472SVG
CSB-BP311472SVG
CSB-MP311472SVG
CSB-EP311472SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas oleovorans Outer membrane protein AlkL (alkL) CSB-YP311473PUQ
CSB-EP311473PUQ
CSB-BP311473PUQ
CSB-MP311473PUQ
CSB-EP311473PUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pheromone P-factor receptor (mam2), partial CSB-YP311474SXV1
CSB-EP311474SXV1
CSB-BP311474SXV1
CSB-MP311474SXV1
CSB-EP311474SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Prothyroliberin type B (trh-b) CSB-YP311475XBE
CSB-EP311475XBE
CSB-BP311475XBE
CSB-MP311475XBE
CSB-EP311475XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmania guyanensis Leishmanolysin (gp63) CSB-YP311476LDP
CSB-EP311476LDP
CSB-BP311476LDP
CSB-MP311476LDP
CSB-EP311476LDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Major capsid protein, partial CSB-YP311477SRG
CSB-EP311477SRG
CSB-BP311477SRG
CSB-MP311477SRG
CSB-EP311477SRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Histone H2B type 1 CSB-YP311478RA
CSB-EP311478RA
CSB-BP311478RA
CSB-MP311478RA
CSB-EP311478RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor 38 (PRP38) CSB-YP311479SVG
CSB-EP311479SVG
CSB-BP311479SVG
CSB-MP311479SVG
CSB-EP311479SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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