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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00500 (AtMg00500) CSB-YP310914DOA
CSB-EP310914DOA
CSB-BP310914DOA
CSB-MP310914DOA
CSB-EP310914DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L2, mitochondrial (RPL2) CSB-YP310915DOA
CSB-EP310915DOA
CSB-BP310915DOA
CSB-MP310915DOA
CSB-EP310915DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Actin-93 CSB-YP310917NHE
CSB-EP310917NHE
CSB-BP310917NHE
CSB-MP310917NHE
CSB-EP310917NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Actin-104 CSB-YP310918NHE
CSB-EP310918NHE
CSB-BP310918NHE
CSB-MP310918NHE
CSB-EP310918NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Superoxide dismutase [Cu-Zn], chloroplastic (SODCP) CSB-YP310919OFG
CSB-EP310919OFG
CSB-BP310919OFG
CSB-MP310919OFG
CSB-EP310919OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica S-adenosylmethionine synthase 2 (SAM2) CSB-YP310920OFG
CSB-EP310920OFG
CSB-BP310920OFG
CSB-MP310920OFG
CSB-EP310920OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Styphnolobium japonicum Bark lectin CSB-YP310921SRB
CSB-EP310921SRB
CSB-BP310921SRB
CSB-MP310921SRB
CSB-EP310921SRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Guanine nucleotide-binding protein subunit beta (GB1) CSB-YP310922FIG
CSB-EP310922FIG
CSB-BP310922FIG
CSB-MP310922FIG
CSB-EP310922FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Actin-82 CSB-YP310923FIG
CSB-EP310923FIG
CSB-BP310923FIG
CSB-MP310923FIG
CSB-EP310923FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Serpin-Z2B CSB-YP310924TQN
CSB-EP310924TQN
CSB-BP310924TQN
CSB-MP310924TQN
CSB-EP310924TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Valine--tRNA ligase (VALRS), partial CSB-YP310925DOA
CSB-EP310925DOA
CSB-BP310925DOA
CSB-MP310925DOA
CSB-EP310925DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 30 (At2g41900), partial CSB-YP310926DOA
CSB-EP310926DOA
CSB-BP310926DOA
CSB-MP310926DOA
CSB-EP310926DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At1g04390 (At1g04390), partial CSB-YP310927DOA
CSB-EP310927DOA
CSB-BP310927DOA
CSB-MP310927DOA
CSB-EP310927DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamarindus indica Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP310928TAP
CSB-EP310928TAP
CSB-BP310928TAP
CSB-MP310928TAP
CSB-EP310928TAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP310929FBG
CSB-EP310929FBG
CSB-BP310929FBG
CSB-MP310929FBG
CSB-EP310929FBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic (RBCMT), partial CSB-YP310930NHE
CSB-EP310930NHE
CSB-BP310930NHE
CSB-MP310930NHE
CSB-EP310930NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fatty acyl-CoA reductase (acr1) CSB-YP310931ABT
CSB-EP310931ABT
CSB-BP310931ABT
CSB-MP310931ABT
CSB-EP310931ABT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Hydrogenase nickel incorporation protein HypA (hypA) CSB-YP310932FPZ
CSB-EP310932FPZ
CSB-BP310932FPZ
CSB-MP310932FPZ
CSB-EP310932FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric transport protein (merT), partial CSB-YP310933AGP1
CSB-EP310933AGP1
CSB-BP310933AGP1
CSB-MP310933AGP1
CSB-EP310933AGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alginate biosynthesis protein Alg44 (alg44) CSB-YP310934DPX
CSB-EP310934DPX
CSB-BP310934DPX
CSB-MP310934DPX
CSB-EP310934DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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