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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Calpain-like protease 1 (RIM13), partial CSB-YP312737SVG
CSB-EP312737SVG
CSB-BP312737SVG
CSB-MP312737SVG
CSB-EP312737SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Regulatory protein recX (recX) CSB-YP312738LAN
CSB-EP312738LAN
CSB-BP312738LAN
CSB-MP312738LAN
CSB-EP312738LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Threonine--tRNA ligase (thrS), partial CSB-YP312739LAN
CSB-EP312739LAN
CSB-BP312739LAN
CSB-MP312739LAN
CSB-EP312739LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription activator MSS11 (MSS11), partial CSB-YP312740SVG
CSB-EP312740SVG
CSB-BP312740SVG
CSB-MP312740SVG
CSB-EP312740SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Monothiol glutaredoxin-3 (GRX3) CSB-YP312741SVG
CSB-EP312741SVG
CSB-BP312741SVG
CSB-MP312741SVG
CSB-EP312741SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-DR1 Gag polyprotein (TY1A-DR1) CSB-YP312742SVG
CSB-EP312742SVG
CSB-BP312742SVG
CSB-MP312742SVG
CSB-EP312742SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR102C (YDR102C) CSB-YP312743SVG
CSB-EP312743SVG
CSB-BP312743SVG
CSB-MP312743SVG
CSB-EP312743SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YIR017W-A (YIR017W-A), partial CSB-YP312744SVG1
CSB-EP312744SVG1
CSB-BP312744SVG1
CSB-MP312744SVG1
CSB-EP312744SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae F-box protein YDR131C (YDR131C), partial CSB-YP312745SVG
CSB-EP312745SVG
CSB-BP312745SVG
CSB-MP312745SVG
CSB-EP312745SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Ribosome maturation factor RimM (rimM) CSB-YP312746LAN
CSB-EP312746LAN
CSB-BP312746LAN
CSB-MP312746LAN
CSB-EP312746LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Ribosome maturation factor RimP (rimP) CSB-YP312747LAN
CSB-EP312747LAN
CSB-BP312747LAN
CSB-MP312747LAN
CSB-EP312747LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Elongation factor 4 (lepA), partial CSB-YP312748LAN
CSB-EP312748LAN
CSB-BP312748LAN
CSB-MP312748LAN
CSB-EP312748LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Formate--tetrahydrofolate ligase (fhs), partial CSB-YP312749LAN
CSB-EP312749LAN
CSB-BP312749LAN
CSB-MP312749LAN
CSB-EP312749LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae eRF1 methyltransferase catalytic subunit MTQ2 (MTQ2) CSB-YP312750SVG
CSB-EP312750SVG
CSB-BP312750SVG
CSB-MP312750SVG
CSB-EP312750SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae Invasin ipaC (ipaC) CSB-YP312751SZA
CSB-EP312751SZA
CSB-BP312751SZA
CSB-MP312751SZA
CSB-EP312751SZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of V-ATPase in vacuolar membrane protein 2 (RAV2) CSB-YP312752SVG
CSB-EP312752SVG
CSB-BP312752SVG
CSB-MP312752SVG
CSB-EP312752SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transposon Ty1-DR2 Gag polyprotein (TY1A-DR2) CSB-YP312753SVG
CSB-EP312753SVG
CSB-BP312753SVG
CSB-MP312753SVG
CSB-EP312753SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lytechinus pictus Calcium-binding protein LPS1-beta CSB-YP312754LRW
CSB-EP312754LRW
CSB-BP312754LRW
CSB-MP312754LRW
CSB-EP312754LRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP312755LAN
CSB-EP312755LAN
CSB-BP312755LAN
CSB-MP312755LAN
CSB-EP312755LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Trigger factor (tig) CSB-YP312756LAN
CSB-EP312756LAN
CSB-BP312756LAN
CSB-MP312756LAN
CSB-EP312756LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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