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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter aerogenes Quaternary ammonium compound-resistance protein qacE (qacE), partial CSB-YP314590EKN1
CSB-EP314590EKN1
CSB-BP314590EKN1
CSB-MP314590EKN1
CSB-EP314590EKN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Fe] (sodB) CSB-YP314591EGX
CSB-EP314591EGX
CSB-BP314591EGX
CSB-MP314591EGX
CSB-EP314591EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yieF (yieF) CSB-YP314592SZB
CSB-EP314592SZB
CSB-BP314592SZB
CSB-MP314592SZB
CSB-EP314592SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-threonine dehydratase catabolic TdcB (tdcB) CSB-YP314593ENV
CSB-EP314593ENV
CSB-BP314593ENV
CSB-MP314593ENV
CSB-EP314593ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiamine-monophosphate kinase (thiL) CSB-YP314594EGX
CSB-EP314594EGX
CSB-BP314594EGX
CSB-MP314594EGX
CSB-EP314594EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein tldD (tldD) CSB-YP314595EGX
CSB-EP314595EGX
CSB-BP314595EGX
CSB-MP314595EGX
CSB-EP314595EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trk system potassium uptake protein trkA (trkA) CSB-YP314596EGX
CSB-EP314596EGX
CSB-BP314596EGX
CSB-MP314596EGX
CSB-EP314596EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tyrosine--tRNA ligase (tyrS) CSB-YP314597ENV
CSB-EP314597ENV
CSB-BP314597ENV
CSB-MP314597ENV
CSB-EP314597ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator iscR (iscR) CSB-YP314598ENV
CSB-EP314598ENV
CSB-BP314598ENV
CSB-MP314598ENV
CSB-EP314598ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase E (rsmE) CSB-YP314599EGX
CSB-EP314599EGX
CSB-BP314599EGX
CSB-MP314599EGX
CSB-EP314599EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uracil permease (uraA), partial CSB-YP314600ENV
CSB-EP314600ENV
CSB-BP314600ENV
CSB-MP314600ENV
CSB-EP314600ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine permease XanP (xanP), partial CSB-YP314601EGX
CSB-EP314601EGX
CSB-BP314601EGX
CSB-MP314601EGX
CSB-EP314601EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Dihydropteroate synthase type-1 (sulI) CSB-YP314602CQJ
CSB-EP314602CQJ
CSB-BP314602CQJ
CSB-MP314602CQJ
CSB-EP314602CQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP314603EGX
CSB-EP314603EGX
CSB-BP314603EGX
CSB-MP314603EGX
CSB-EP314603EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nudix hydrolase 24, chloroplastic (NUDT24) CSB-YP314604DOA
CSB-EP314604DOA
CSB-BP314604DOA
CSB-MP314604DOA
CSB-EP314604DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein essA (essA), partial CSB-YP314605FKZ
CSB-EP314605FKZ
CSB-BP314605FKZ
CSB-MP314605FKZ
CSB-EP314605FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small heat shock protein ibpA (ibpA) CSB-YP314606SZB
CSB-EP314606SZB
CSB-BP314606SZB
CSB-MP314606SZB
CSB-EP314606SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneurinibacillus migulanus Gramicidin S synthase 1 (grsA), partial CSB-YP314607AKF
CSB-EP314607AKF
CSB-BP314607AKF
CSB-MP314607AKF
CSB-EP314607AKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Multidrug/solvent efflux pump membrane transporter MepB (mepB), partial CSB-YP314608FFZ
CSB-EP314608FFZ
CSB-BP314608FFZ
CSB-MP314608FFZ
CSB-EP314608FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant mRNA interferase RelE (relE) CSB-YP314609SZB
CSB-EP314609SZB
CSB-BP314609SZB
CSB-MP314609SZB
CSB-EP314609SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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