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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Bifunctional protein BirA (birA) CSB-YP315604BRJ
CSB-EP315604BRJ
CSB-BP315604BRJ
CSB-MP315604BRJ
CSB-EP315604BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 50S ribosomal protein L24 (rplX) CSB-YP315605BRJ
CSB-EP315605BRJ
CSB-BP315605BRJ
CSB-MP315605BRJ
CSB-EP315605BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Thymidylate synthase 1 (thyA1) CSB-YP315606BRJ
CSB-EP315606BRJ
CSB-BP315606BRJ
CSB-MP315606BRJ
CSB-EP315606BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Putative neurotoxin-D CSB-YP315607LRM
CSB-EP315607LRM
CSB-BP315607LRM
CSB-MP315607LRM
CSB-EP315607LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Putative calcium channel toxin 196 CSB-YP315608LRM
CSB-EP315608LRM
CSB-BP315608LRM
CSB-MP315608LRM
CSB-EP315608LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Antimicrobial peptide 36.4 CSB-YP315609LRM
CSB-EP315609LRM
CSB-BP315609LRM
CSB-MP315609LRM
CSB-EP315609LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Anionic peptide 10.1 CSB-YP315610LRM
CSB-EP315610LRM
CSB-BP315610LRM
CSB-MP315610LRM
CSB-EP315610LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Neurotoxin 60.35 CSB-YP315611LRM
CSB-EP315611LRM
CSB-BP315611LRM
CSB-MP315611LRM
CSB-EP315611LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 27.1 CSB-YP315612LRM
CSB-EP315612LRM
CSB-BP315612LRM
CSB-MP315612LRM
CSB-EP315612LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 214 CSB-YP315613LRM
CSB-EP315613LRM
CSB-BP315613LRM
CSB-MP315613LRM
CSB-EP315613LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 164 CSB-YP315614LRM
CSB-EP315614LRM
CSB-BP315614LRM
CSB-MP315614LRM
CSB-EP315614LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lychas mucronatus Venom protein 30.1 CSB-YP315615LRM
CSB-EP315615LRM
CSB-BP315615LRM
CSB-MP315615LRM
CSB-EP315615LRM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isometrus maculatus Potassium channel toxin ImKTx88 CSB-YP315616INI
CSB-EP315616INI
CSB-BP315616INI
CSB-MP315616INI
CSB-EP315616INI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaphus truei Ascaphin-3 CSB-YP315617APC
CSB-EP315617APC
CSB-BP315617APC
CSB-MP315617APC
CSB-EP315617APC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaphus truei Ascaphin-7 CSB-YP315618APC
CSB-EP315618APC
CSB-BP315618APC
CSB-MP315618APC
CSB-EP315618APC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eumenes rubrofemoratus Eumenitin-R CSB-YP315619EIA
CSB-EP315619EIA
CSB-BP315619EIA
CSB-MP315619EIA
CSB-EP315619EIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Actin-1 (ACT1) CSB-YP315620DOA
CSB-EP315620DOA
CSB-BP315620DOA
CSB-MP315620DOA
CSB-EP315620DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 46 (CRRSP46) CSB-YP315621DOA
CSB-EP315621DOA
CSB-BP315621DOA
CSB-MP315621DOA
CSB-EP315621DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 50 (CRRSP50) CSB-YP315622DOA
CSB-EP315622DOA
CSB-BP315622DOA
CSB-MP315622DOA
CSB-EP315622DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. kurstaki N-acyl homoserine lactonase AiiA (aiiA) CSB-YP315623BDC
CSB-BP315623BDC
CSB-MP315623BDC
CSB-EP315623BDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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