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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant D-xylose-proton symporter (xylE), partial CSB-YP314857EOD
CSB-EP314857EOD
CSB-BP314857EOD
CSB-MP314857EOD
CSB-EP314857EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein tldD (tldD) CSB-YP314859SZB
CSB-EP314859SZB
CSB-BP314859SZB
CSB-MP314859SZB
CSB-EP314859SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribose transport system permease protein rbsC (rbsC), partial CSB-YP314860ENV1
CSB-EP314860ENV1
CSB-BP314860ENV1
CSB-MP314860ENV1
CSB-EP314860ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose transport system permease protein xylH (xylH), partial CSB-YP314861EGX
CSB-EP314861EGX
CSB-BP314861EGX
CSB-MP314861EGX
CSB-EP314861EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trk system potassium uptake protein trkA (trkA) CSB-YP314862EOD
CSB-EP314862EOD
CSB-BP314862EOD
CSB-MP314862EOD
CSB-EP314862EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized tRNA/rRNA methyltransferase YfiF (yfiF) CSB-YP314863ENV
CSB-EP314863ENV
CSB-BP314863ENV
CSB-MP314863ENV
CSB-EP314863ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA-binding protein YhbY (yhbY) CSB-YP314864SZB
CSB-EP314864SZB
CSB-BP314864SZB
CSB-MP314864SZB
CSB-EP314864SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome association toxin RatA (ratA) CSB-YP314865ENV
CSB-EP314865ENV
CSB-BP314865ENV
CSB-MP314865ENV
CSB-EP314865ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0118 inner membrane protein yhhT (yhhT), partial CSB-YP314866EOD
CSB-EP314866EOD
CSB-BP314866EOD
CSB-MP314866EOD
CSB-EP314866EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil permease (uraA), partial CSB-YP314867EOD
CSB-EP314867EOD
CSB-BP314867EOD
CSB-MP314867EOD
CSB-EP314867EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halocynthia aurantium Dicynthaurin CSB-YP314868HBQ
CSB-EP314868HBQ
CSB-BP314868HBQ
CSB-MP314868HBQ
CSB-EP314868HBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP314869SZB
CSB-EP314869SZB
CSB-BP314869SZB
CSB-MP314869SZB
CSB-EP314869SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Diphosphoinositol polyphosphate phosphohydrolase 3-beta (Nudt11) CSB-YP314870MO
CSB-EP314870MO
CSB-BP314870MO
CSB-MP314870MO
CSB-EP314870MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL60 (ATL60), partial CSB-YP314871DOA1
CSB-EP314871DOA1
CSB-BP314871DOA1
CSB-MP314871DOA1
CSB-EP314871DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0345 protein YaiE (yaiE) CSB-YP314872EOD
CSB-EP314872EOD
CSB-BP314872EOD
CSB-MP314872EOD
CSB-EP314872EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Type VII secretion system extracellular protein B (esxB) CSB-YP314873FLG
CSB-BP314873FLG
CSB-MP314873FLG
CSB-EP314873FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein essB (essB), partial CSB-YP314874FKZ
CSB-EP314874FKZ
CSB-BP314874FKZ
CSB-MP314874FKZ
CSB-EP314874FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small heat shock protein ibpA (ibpA) CSB-YP314875EOD
CSB-EP314875EOD
CSB-BP314875EOD
CSB-MP314875EOD
CSB-EP314875EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aneurinibacillus migulanus Gramicidin S synthase 2 (grsB), partial CSB-YP314876AKF
CSB-EP314876AKF
CSB-BP314876AKF
CSB-MP314876AKF
CSB-EP314876AKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoid occlusion factor SlmA (slmA) CSB-YP314877ENV
CSB-EP314877ENV
CSB-BP314877ENV
CSB-MP314877ENV
CSB-EP314877ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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