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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae 40S ribosomal protein S22-A (RPS22A) CSB-YP314900SVG
CSB-EP314900SVG
CSB-BP314900SVG
CSB-MP314900SVG
CSB-EP314900SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L11-A (RPL11A) CSB-YP314901SVG
CSB-EP314901SVG
CSB-BP314901SVG
CSB-MP314901SVG
CSB-EP314901SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Chlorophyllide reductase subunit Z (bchZ) CSB-YP314902RLE
CSB-EP314902RLE
CSB-BP314902RLE
CSB-MP314902RLE
CSB-EP314902RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant?Davidiella tassiana NADP-dependent mannitol dehydrogenase (P0C0Y5) CSB-YP314903DAY
CSB-EP314903DAY
CSB-BP314903DAY
CSB-MP314903DAY
CSB-EP314903DAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 UvrABC system protein A (uvrA), partial CSB-YP314904BWS
CSB-EP314904BWS
CSB-BP314904BWS
CSB-MP314904BWS
CSB-EP314904BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP314905BWS
CSB-EP314905BWS
CSB-BP314905BWS
CSB-MP314905BWS
CSB-EP314905BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Adenylosuccinate synthetase (purA) CSB-YP314906BWS
CSB-EP314906BWS
CSB-BP314906BWS
CSB-MP314906BWS
CSB-EP314906BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Urease subunit gamma 1 (ureA1) CSB-YP314907BWS
CSB-EP314907BWS
CSB-BP314907BWS
CSB-MP314907BWS
CSB-EP314907BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Protein-export protein SecB (secB) CSB-YP314908BWS
CSB-EP314908BWS
CSB-BP314908BWS
CSB-MP314908BWS
CSB-EP314908BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin-responsive protein IAA31 (IAA31) CSB-YP314909OFG
CSB-EP314909OFG
CSB-BP314909OFG
CSB-MP314909OFG
CSB-EP314909OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Nuclear export protein (NS) CSB-YP314910IMY
CSB-EP314910IMY
CSB-BP314910IMY
CSB-MP314910IMY
CSB-EP314910IMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Uncharacterized protein AN0679 (AN10108) CSB-YP314911EKF
CSB-EP314911EKF
CSB-BP314911EKF
CSB-MP314911EKF
CSB-EP314911EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides elegans Potassium channel toxin alpha-KTx 2.8 CSB-YP314912CFW
CSB-EP314912CFW
CSB-BP314912CFW
CSB-MP314912CFW
CSB-EP314912CFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anuroctonus phaiodactylus Potassium channel toxin alpha-KTx 6.12 CSB-YP314913ALW
CSB-EP314913ALW
CSB-BP314913ALW
CSB-MP314913ALW
CSB-EP314913ALW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Histone H2A type 1-E CSB-YP314914RA
CSB-EP314914RA
CSB-BP314914RA
CSB-MP314914RA
CSB-EP314914RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plastocyanin (petE) CSB-YP314915AJN
CSB-EP314915AJN
CSB-BP314915AJN
CSB-MP314915AJN
CSB-EP314915AJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P) transhydrogenase subunit alpha part 1 CSB-YP314916RMA
CSB-EP314916RMA
CSB-BP314916RMA
CSB-MP314916RMA
CSB-EP314916RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Light-harvesting protein B-870 beta chain, partial CSB-YP314917RMA
CSB-EP314917RMA
CSB-BP314917RMA
CSB-MP314917RMA
CSB-EP314917RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dickeya dadantii Pectate lyase E (pelE) CSB-YP314918DKD
CSB-EP314918DKD
CSB-BP314918DKD
CSB-MP314918DKD
CSB-EP314918DKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabuthus transvaalicus Ikitoxin CSB-YP314919PBV
CSB-EP314919PBV
CSB-BP314919PBV
CSB-MP314919PBV
CSB-EP314919PBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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