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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vipera ammodytes ammodytes Factor X-activator 2 light chain 2 (LC2) CSB-YP316435VFO
CSB-EP316435VFO
CSB-BP316435VFO
CSB-MP316435VFO
CSB-EP316435VFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa 60S ribosomal protein L2, mitochondrial (RPL2), partial CSB-YP316436OCO
CSB-EP316436OCO
CSB-BP316436OCO
CSB-MP316436OCO
CSB-EP316436OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g26485 (At4g26485) CSB-YP316437DOA
CSB-EP316437DOA
CSB-BP316437DOA
CSB-MP316437DOA
CSB-EP316437DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Cytochrome b6 (petB), partial CSB-YP316438TDQ1
CSB-EP316438TDQ1
CSB-BP316438TDQ1
CSB-MP316438TDQ1
CSB-EP316438TDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Apocytochrome f (petA), partial CSB-YP316439TDQ1
CSB-EP316439TDQ1
CSB-BP316439TDQ1
CSB-MP316439TDQ1
CSB-EP316439TDQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus tRNA modification GTPase MnmE (mnmE) CSB-YP316440TDQ
CSB-EP316440TDQ
CSB-BP316440TDQ
CSB-MP316440TDQ
CSB-EP316440TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiostrepton (tpdA) CSB-YP316441FNX
CSB-EP316441FNX
CSB-BP316441FNX
CSB-MP316441FNX
CSB-EP316441FNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance RPP8-like protein 2 (RPP8L2), partial CSB-YP316442DOA
CSB-EP316442DOA
CSB-BP316442DOA
CSB-MP316442DOA
CSB-EP316442DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana picturata Brevinin-1PTa CSB-YP316443RBD
CSB-EP316443RBD
CSB-BP316443RBD
CSB-MP316443RBD
CSB-EP316443RBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana hosii Nigrocin-2HSa CSB-YP316444RAZ
CSB-EP316444RAZ
CSB-BP316444RAZ
CSB-MP316444RAZ
CSB-EP316444RAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus stercusmuscarum Alpha-conotoxin-like Sm1.1 CSB-YP316445CPG
CSB-EP316445CPG
CSB-BP316445CPG
CSB-MP316445CPG
CSB-EP316445CPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadrurus gertschi Non-disulfide-bridged peptide 5.6 CSB-YP316446HST
CSB-EP316446HST
CSB-BP316446HST
CSB-MP316446HST
CSB-EP316446HST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Peptide 4913 CSB-YP316447TEV
CSB-EP316447TEV
CSB-BP316447TEV
CSB-MP316447TEV
CSB-EP316447TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadrurus gertschi Potassium channel toxin alpha-KTx CSB-YP316448HST
CSB-EP316448HST
CSB-BP316448HST
CSB-MP316448HST
CSB-EP316448HST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g28640 (At1g28640) CSB-YP316449DOA
CSB-EP316449DOA
CSB-BP316449DOA
CSB-MP316449DOA
CSB-EP316449DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Chaperone protein DnaK (dnaK), partial CSB-YP316450BUD
CSB-EP316450BUD
CSB-BP316450BUD
CSB-MP316450BUD
CSB-EP316450BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesis muta muta Phospholipase A2 LM-PLA2-II CSB-YP316451LMC
CSB-EP316451LMC
CSB-BP316451LMC
CSB-MP316451LMC
CSB-EP316451LMC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Chaperone protein htpG (htpG), partial CSB-YP316452EYA
CSB-EP316452EYA
CSB-BP316452EYA
CSB-MP316452EYA
CSB-EP316452EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small COPII coat GTPase SAR1 (sar1) CSB-YP316453DOZ
CSB-EP316453DOZ
CSB-BP316453DOZ
CSB-MP316453DOZ
CSB-EP316453DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata 1,3-beta-glucanosyltransferase gel4 (gel4) CSB-BP316454NGS
CSB-MP316454NGS
CSB-EP316454NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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