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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized protein At3g50808 (At3g50808) CSB-YP316535DOA
CSB-EP316535DOA
CSB-BP316535DOA
CSB-MP316535DOA
CSB-EP316535DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH131 (BHLH131) CSB-YP316536DOA
CSB-EP316536DOA
CSB-BP316536DOA
CSB-MP316536DOA
CSB-EP316536DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP316537FMW
CSB-EP316537FMW
CSB-BP316537FMW
CSB-MP316537FMW
CSB-EP316537FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Golgi to ER traffic protein 2 (GET2), partial CSB-YP316538CZD1
CSB-EP316538CZD1
CSB-BP316538CZD1
CSB-MP316538CZD1
CSB-EP316538CZD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial (NDUFC1), partial CSB-YP316539EXP
CSB-EP316539EXP
CSB-BP316539EXP
CSB-MP316539EXP
CSB-EP316539EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotate phosphoribosyltransferase (pyrE) CSB-YP316540FMW
CSB-EP316540FMW
CSB-BP316540FMW
CSB-MP316540FMW
CSB-EP316540FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (NDUFA10) CSB-YP316541EXP
CSB-EP316541EXP
CSB-BP316541EXP
CSB-MP316541EXP
CSB-EP316541EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Transmembrane domain-containing protein DDB_G0287209 (DDB_G0287209), partial CSB-YP316542DKK
CSB-EP316542DKK
CSB-BP316542DKK
CSB-MP316542DKK
CSB-EP316542DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Osmia rufa Osmin CSB-YP316543OCR
CSB-EP316543OCR
CSB-BP316543OCR
CSB-MP316543OCR
CSB-EP316543OCR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP316544DSB
CSB-EP316544DSB
CSB-BP316544DSB
CSB-MP316544DSB
CSB-EP316544DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 50S ribosomal protein L29 (rpmC) CSB-YP316545DSB
CSB-EP316545DSB
CSB-BP316545DSB
CSB-MP316545DSB
CSB-EP316545DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ymjD (ymjD) CSB-YP316546ENV
CSB-EP316546ENV
CSB-BP316546ENV
CSB-MP316546ENV
CSB-EP316546ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YER039C-A (YER039C-A), partial CSB-YP316547SVG1
CSB-EP316547SVG1
CSB-BP316547SVG1
CSB-MP316547SVG1
CSB-EP316547SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 30S ribosomal protein S10 (rpsJ) CSB-YP316548DSB
CSB-EP316548DSB
CSB-BP316548DSB
CSB-MP316548DSB
CSB-EP316548DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 3-deoxy-D-manno-octulosonic-acid transferase (waaA), partial CSB-YP316549DSB
CSB-EP316549DSB
CSB-BP316549DSB
CSB-MP316549DSB
CSB-EP316549DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Virulence plasmid protein pGP3-D CSB-YP316550DSA
CSB-BP316550DSA
CSB-MP316550DSA
CSB-EP316550DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus capitaneus Alpha-conotoxin-like Cp20.2 CSB-YP316551DVV
CSB-EP316551DVV
CSB-BP316551DVV
CSB-MP316551DVV
CSB-EP316551DVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus miles Alpha-conotoxin-like Ml20.2 CSB-YP316552DWE
CSB-EP316552DWE
CSB-BP316552DWE
CSB-MP316552DWE
CSB-EP316552DWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5K (insH8) CSB-YP316553ENV
CSB-EP316553ENV
CSB-BP316553ENV
CSB-MP316553ENV
CSB-EP316553ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5-14 (JW5914) CSB-YP316554ENV
CSB-EP316554ENV
CSB-BP316554ENV
CSB-MP316554ENV
CSB-EP316554ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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