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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Quinic acid utilization activator (qa-1f), partial CSB-YP318159NHA
CSB-EP318159NHA
CSB-BP318159NHA
CSB-MP318159NHA
CSB-EP318159NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize chlorotic mottle virus Uncharacterized protein p31 (ORF3) CSB-YP318160MBG
CSB-EP318160MBG
CSB-BP318160MBG
CSB-MP318160MBG
CSB-EP318160MBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylmalonyl-CoA mutase large subunit (mutB), partial CSB-YP318161PSH
CSB-EP318161PSH
CSB-BP318161PSH
CSB-MP318161PSH
CSB-EP318161PSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yggD (yggD) CSB-YP318162ENV
CSB-EP318162ENV
CSB-BP318162ENV
CSB-MP318162ENV
CSB-EP318162ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Basic form of pathogenesis-related protein 1 CSB-YP318163NHE
CSB-EP318163NHE
CSB-BP318163NHE
CSB-MP318163NHE
CSB-EP318163NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus Movement protein CSB-YP318164TGA
CSB-EP318164TGA
CSB-BP318164TGA
CSB-MP318164TGA
CSB-EP318164TGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antibacterial protein 2 CSB-YP318165SLF
CSB-EP318165SLF
CSB-BP318165SLF
CSB-MP318165SLF
CSB-EP318165SLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 8 CSB-YP318167LHY
CSB-EP318167LHY
CSB-BP318167LHY
CSB-MP318167LHY
CSB-EP318167LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 64 CSB-YP318168LHY
CSB-EP318168LHY
CSB-BP318168LHY
CSB-MP318168LHY
CSB-EP318168LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methylated-DNA--protein-cysteine methyltransferase, constitutive (ogt) CSB-YP318169BRJ
CSB-EP318169BRJ
CSB-BP318169BRJ
CSB-MP318169BRJ
CSB-EP318169BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Patatin group M-1 CSB-YP318170FIG
CSB-EP318170FIG
CSB-BP318170FIG
CSB-MP318170FIG
CSB-EP318170FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 41 Early E2A DNA-binding protein (DBP) CSB-YP318171HIS
CSB-EP318171HIS
CSB-BP318171HIS
CSB-MP318171HIS
CSB-EP318171HIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 Hexon protein (L3), partial CSB-YP318172HIR
CSB-BP318172HIR
CSB-MP318172HIR
CSB-EP318172HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 Adenain CSB-YP318173HIR
CSB-EP318173HIR
CSB-BP318173HIR
CSB-MP318173HIR
CSB-EP318173HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nitrate reductase [NADH] 1 (NIA1), partial CSB-YP318174DOA
CSB-EP318174DOA
CSB-BP318174DOA
CSB-MP318174DOA
CSB-EP318174DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Reaction center protein M chain (pufM), partial CSB-YP318175RLC1
CSB-EP318175RLC1
CSB-BP318175RLC1
CSB-MP318175RLC1
CSB-EP318175RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP318176ROL
CSB-EP318176ROL
CSB-BP318176ROL
CSB-MP318176ROL
CSB-EP318176ROL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Triose phosphate/phosphate translocator, chloroplastic, partial CSB-YP318177FKI1
CSB-EP318177FKI1
CSB-BP318177FKI1
CSB-MP318177FKI1
CSB-EP318177FKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant High mobility group protein C CSB-YP318178TLU
CSB-EP318178TLU
CSB-BP318178TLU
CSB-MP318178TLU
CSB-EP318178TLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (murF) CSB-YP318179ENV
CSB-EP318179ENV
CSB-BP318179ENV
CSB-MP318179ENV
CSB-EP318179ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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