Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Small COPII coat GTPase SAR1 (SAR1) CSB-YP317342CTK
CSB-EP317342CTK
CSB-BP317342CTK
CSB-MP317342CTK
CSB-EP317342CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 3-ketoacyl-CoA reductase (CNI00690), partial CSB-YP317343CTL1
CSB-EP317343CTL1
CSB-BP317343CTL1
CSB-MP317343CTL1
CSB-EP317343CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein transport protein SEC24 (SEC24), partial CSB-YP317344CTL
CSB-EP317344CTL
CSB-BP317344CTL
CSB-MP317344CTL
CSB-EP317344CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Sensitive to high expression protein 9 homolog, mitochondrial (SHE9), partial CSB-YP317345CTL
CSB-EP317345CTL
CSB-BP317345CTL
CSB-MP317345CTL
CSB-EP317345CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-processing protein 45 (PRP45), partial CSB-YP317346CTL
CSB-EP317346CTL
CSB-BP317346CTL
CSB-MP317346CTL
CSB-EP317346CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Sorting nexin-41 (SNX41), partial CSB-YP317347CTL
CSB-EP317347CTL
CSB-BP317347CTL
CSB-MP317347CTL
CSB-EP317347CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D FACT complex subunit POB3 (POB3), partial CSB-YP317348CTK
CSB-EP317348CTK
CSB-BP317348CTK
CSB-MP317348CTK
CSB-EP317348CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Translation machinery-associated protein 22 (TMA22) CSB-YP317349CTL
CSB-EP317349CTL
CSB-BP317349CTL
CSB-MP317349CTL
CSB-EP317349CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM54 (TIM54), partial CSB-YP317350CTK1
CSB-EP317350CTK1
CSB-BP317350CTK1
CSB-MP317350CTK1
CSB-EP317350CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRM61 CSB-YP317351CTK
CSB-EP317351CTK
CSB-BP317351CTK
CSB-MP317351CTK
CSB-EP317351CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D DNA damage-inducible protein 1 (DDI1) CSB-YP317352CTL
CSB-EP317352CTL
CSB-BP317352CTL
CSB-MP317352CTL
CSB-EP317352CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Plasma membrane proteolipid 3 (PMP3), partial CSB-YP317353CTK
CSB-EP317353CTK
CSB-BP317353CTK
CSB-MP317353CTK
CSB-EP317353CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Urease (URE1), partial CSB-YP317354CTL
CSB-EP317354CTL
CSB-BP317354CTL
CSB-MP317354CTL
CSB-EP317354CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar protein sorting-associated protein 27 (VPS27), partial CSB-YP317355CTK
CSB-EP317355CTK
CSB-BP317355CTK
CSB-MP317355CTK
CSB-EP317355CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone acetyltransferase type B subunit 2 (HAT2) CSB-YP317356CTL
CSB-EP317356CTL
CSB-BP317356CTL
CSB-MP317356CTL
CSB-EP317356CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein HIR1 (HIR1), partial CSB-YP317357CTK
CSB-EP317357CTK
CSB-BP317357CTK
CSB-MP317357CTK
CSB-EP317357CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial division protein 1 (MDV1), partial CSB-YP317358CTL
CSB-EP317358CTL
CSB-BP317358CTL
CSB-MP317358CTL
CSB-EP317358CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP317359CTK
CSB-EP317359CTK
CSB-BP317359CTK
CSB-MP317359CTK
CSB-EP317359CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribosome biogenesis protein YTM1 (YTM1) CSB-YP317360CTL
CSB-EP317360CTL
CSB-BP317360CTL
CSB-MP317360CTL
CSB-EP317360CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D pH-response transcription factor pacC/RIM101 (RIM101), partial CSB-YP317361CTK
CSB-EP317361CTK
CSB-BP317361CTK
CSB-MP317361CTK
CSB-EP317361CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>