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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Influenza B virus Hemagglutinin (HA) CSB-YP318240IJJ
CSB-EP318240IJJ
CSB-BP318240IJJ
CSB-MP318240IJJ
CSB-EP318240IJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase acidic protein (PA), partial CSB-YP318241IEE
CSB-EP318241IEE
CSB-BP318241IEE
CSB-MP318241IEE
CSB-EP318241IEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Tat (tat) CSB-YP318242HMA
CSB-EP318242HMA
CSB-BP318242HMA
CSB-MP318242HMA
CSB-EP318242HMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx (vpx) CSB-YP318243HMA
CSB-EP318243HMA
CSB-BP318243HMA
CSB-MP318243HMA
CSB-EP318243HMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella vulgaris Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP318244DSP
CSB-EP318244DSP
CSB-BP318244DSP
CSB-MP318244DSP
CSB-EP318244DSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP318245ROM
CSB-EP318245ROM
CSB-BP318245ROM
CSB-MP318245ROM
CSB-EP318245ROM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Nef (nef), partial CSB-YP318246HLK
CSB-EP318246HLK
CSB-BP318246HLK
CSB-MP318246HLK
CSB-EP318246HLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP318247HKR
CSB-EP318247HKR
CSB-BP318247HKR
CSB-MP318247HKR
CSB-EP318247HKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Envelope glycoprotein gp160 (env), partial CSB-YP318248SGX
CSB-EP318248SGX
CSB-BP318248SGX
CSB-MP318248SGX
CSB-EP318248SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Gag polyprotein (gag) CSB-YP318249HKR
CSB-EP318249HKR
CSB-BP318249HKR
CSB-MP318249HKR
CSB-EP318249HKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Gag-Pol polyprotein (gag-pol), partial CSB-YP318250HLK
CSB-EP318250HLK
CSB-BP318250HLK
CSB-MP318250HLK
CSB-EP318250HLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Virion infectivity factor (vif) CSB-YP318251SGX
CSB-EP318251SGX
CSB-BP318251SGX
CSB-MP318251SGX
CSB-EP318251SGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Tat CSB-YP318252HLM
CSB-EP318252HLM
CSB-BP318252HLM
CSB-MP318252HLM
CSB-EP318252HLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpu (vpu), partial CSB-YP318253HKN
CSB-EP318253HKN
CSB-BP318253HKN
CSB-MP318253HKN
CSB-EP318253HKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable proton ATPase 1B (H1B), partial CSB-YP318254LOE
CSB-EP318254LOE
CSB-BP318254LOE
CSB-MP318254LOE
CSB-EP318254LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P22 Bifunctional tail protein (9), partial CSB-YP318255EDG
CSB-EP318255EDG
CSB-BP318255EDG
CSB-MP318255EDG
CSB-EP318255EDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine adenovirus A serotype 1 E1B protein, small T-antigen CSB-YP318256MUS
CSB-EP318256MUS
CSB-BP318256MUS
CSB-MP318256MUS
CSB-EP318256MUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine adenovirus A serotype 1 Maturation protein (PIVA2) CSB-YP318257MUS
CSB-EP318257MUS
CSB-BP318257MUS
CSB-MP318257MUS
CSB-EP318257MUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Hemagglutinin-neuraminidase (HN), partial CSB-YP318259NCN1
CSB-EP318259NCN1
CSB-BP318259NCN1
CSB-MP318259NCN1
CSB-EP318259NCN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 3 virus Hemagglutinin-neuraminidase (HN), partial CSB-YP318260HPE1
CSB-EP318260HPE1
CSB-BP318260HPE1
CSB-MP318260HPE1
CSB-EP318260HPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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