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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Glucose-6-phosphate 1-dehydrogenase (zwf) CSB-YP318138LPG
CSB-EP318138LPG
CSB-BP318138LPG
CSB-MP318138LPG
CSB-EP318138LPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana acuminata Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP318139NDE
CSB-EP318139NDE
CSB-BP318139NDE
CSB-MP318139NDE
CSB-EP318139NDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Superoxide dismutase [Cu-Zn] 2 (SODCC.1) CSB-YP318140ZAX
CSB-EP318140ZAX
CSB-BP318140ZAX
CSB-MP318140ZAX
CSB-EP318140ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Mandelate racemase (mdlA) CSB-YP318141FFZ
CSB-EP318141FFZ
CSB-BP318141FFZ
CSB-MP318141FFZ
CSB-EP318141FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chlorocatechol 1,2-dioxygenase (clcA) CSB-YP318142FFZ
CSB-EP318142FFZ
CSB-BP318142FFZ
CSB-MP318142FFZ
CSB-EP318142FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio anguillarum Ferric anguibactin-binding protein (fatB) CSB-YP318143VCE
CSB-EP318143VCE
CSB-BP318143VCE
CSB-MP318143VCE
CSB-EP318143VCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein gerE (gerE) CSB-YP318144BRJ
CSB-EP318144BRJ
CSB-BP318144BRJ
CSB-MP318144BRJ
CSB-EP318144BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physarum polycephalum Tubulin alpha-2B chain (ALTBE) CSB-YP318145EVC
CSB-EP318145EVC
CSB-BP318145EVC
CSB-MP318145EVC
CSB-EP318145EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP318146ILK
CSB-EP318146ILK
CSB-BP318146ILK
CSB-MP318146ILK
CSB-EP318146ILK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Plastocyanin minor isoform, chloroplastic (PETE) CSB-YP318147DOA
CSB-EP318147DOA
CSB-BP318147DOA
CSB-MP318147DOA
CSB-EP318147DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metridium senile Metridin CSB-YP318148MTL
CSB-EP318148MTL
CSB-BP318148MTL
CSB-MP318148MTL
CSB-EP318148MTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius DNA-directed RNA polymerase subunit B (rpoB), partial CSB-YP318149FOZ
CSB-EP318149FOZ
CSB-BP318149FOZ
CSB-MP318149FOZ
CSB-EP318149FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius 30S ribosomal protein S12P (rps12p) CSB-YP318150FOZ
CSB-EP318150FOZ
CSB-BP318150FOZ
CSB-MP318150FOZ
CSB-EP318150FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14, chloroplastic (rps14) CSB-YP318151EHU
CSB-EP318151EHU
CSB-BP318151EHU
CSB-MP318151EHU
CSB-EP318151EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein damX (damX), partial CSB-YP318153ENV1
CSB-EP318153ENV1
CSB-BP318153ENV1
CSB-MP318153ENV1
CSB-EP318153ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Methyl-coenzyme M reductase I subunit gamma (mcrG) CSB-YP318154MSQ
CSB-EP318154MSQ
CSB-BP318154MSQ
CSB-MP318154MSQ
CSB-EP318154MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase operon protein D (mcrD) CSB-YP318155MSD
CSB-EP318155MSD
CSB-BP318155MSD
CSB-MP318155MSD
CSB-EP318155MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase PP-Y (PpY-55A) CSB-YP318156DLU
CSB-EP318156DLU
CSB-BP318156DLU
CSB-MP318156DLU
CSB-EP318156DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Protein P1 (ORF1), partial CSB-YP318157PQV1
CSB-EP318157PQV1
CSB-BP318157PQV1
CSB-MP318157PQV1
CSB-EP318157PQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Quinate dehydrogenase (qa-3) CSB-YP318158NHA
CSB-EP318158NHA
CSB-BP318158NHA
CSB-MP318158NHA
CSB-EP318158NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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