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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Human herpesvirus 2 Protein US2 (US2) CSB-YP318343HJX
CSB-EP318343HJX
CSB-BP318343HJX
CSB-MP318343HJX
CSB-EP318343HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nitrogen fixation regulation protein fixK (fixK) CSB-YP318344RKU
CSB-EP318344RKU
CSB-BP318344RKU
CSB-MP318344RKU
CSB-EP318344RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Orotate phosphoribosyltransferase 1 (URA5) CSB-YP318345SVG
CSB-EP318345SVG
CSB-BP318345SVG
CSB-MP318345SVG
CSB-EP318345SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Internal protein III (ipi3) CSB-YP318346EDZ
CSB-EP318346EDZ
CSB-BP318346EDZ
CSB-MP318346EDZ
CSB-EP318346EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 7.2 kDa protein in tk-vs intergenic region (y06A) CSB-YP318347EDZ
CSB-EP318347EDZ
CSB-BP318347EDZ
CSB-MP318347EDZ
CSB-EP318347EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 20.7 kDa protein in vs-regB intergenic region (y06E) CSB-YP318348EDZ
CSB-EP318348EDZ
CSB-BP318348EDZ
CSB-MP318348EDZ
CSB-EP318348EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 6.6 kDa protein in regB-denV intergenic region (y06H) CSB-YP318349EDZ
CSB-EP318349EDZ
CSB-BP318349EDZ
CSB-MP318349EDZ
CSB-EP318349EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Ribonuclease H (rnh) CSB-YP318350EDZ
CSB-EP318350EDZ
CSB-BP318350EDZ
CSB-MP318350EDZ
CSB-EP318350EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 24.6 kDa protein in Gp32-Gp59 intergenic region (y14F) CSB-YP318351EDZ
CSB-EP318351EDZ
CSB-BP318351EDZ
CSB-MP318351EDZ
CSB-EP318351EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 16.0 kDa protein in segB-ipI intergenic region (y07C) CSB-YP318352EDZ
CSB-EP318352EDZ
CSB-BP318352EDZ
CSB-MP318352EDZ
CSB-EP318352EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 10.3 kDa protein in Gp46-Gp47 intergenic region (y03C) CSB-YP318353EDZ
CSB-EP318353EDZ
CSB-BP318353EDZ
CSB-MP318353EDZ
CSB-EP318353EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp28 (28) CSB-YP318354EDZ
CSB-EP318354EDZ
CSB-BP318354EDZ
CSB-MP318354EDZ
CSB-EP318354EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Recombination endonuclease VII (49) CSB-YP318355EDZ
CSB-EP318355EDZ
CSB-BP318355EDZ
CSB-MP318355EDZ
CSB-EP318355EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN3 (CLN3), partial CSB-YP318356SVG
CSB-EP318356SVG
CSB-BP318356SVG
CSB-MP318356SVG
CSB-EP318356SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase, glycosomal (PGI), partial CSB-YP318357TQV
CSB-EP318357TQV
CSB-BP318357TQV
CSB-MP318357TQV
CSB-EP318357TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xiphophorus maculatus Melanoma receptor tyrosine-protein kinase (xmrk), partial CSB-YP318358XAS
CSB-EP318358XAS
CSB-BP318358XAS
CSB-MP318358XAS
CSB-EP318358XAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T5 L-shaped tail fiber protein (ltf), partial CSB-YP318359ELS
CSB-EP318359ELS
CSB-BP318359ELS
CSB-MP318359ELS
CSB-EP318359ELS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eimeria tenella Sporulated oocyst TA4 antigen CSB-YP318360EJU
CSB-EP318360EJU
CSB-BP318360EJU
CSB-MP318360EJU
CSB-EP318360EJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane protein P.IA (porA) CSB-YP318361NGI
CSB-EP318361NGI
CSB-BP318361NGI
CSB-MP318361NGI
CSB-EP318361NGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fimbria A protein (smfA) CSB-YP318362SYN
CSB-EP318362SYN
CSB-BP318362SYN
CSB-MP318362SYN
CSB-EP318362SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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