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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Lithobates catesbeiana Ig light chain C region CSB-YP319982LQA
CSB-EP319982LQA
CSB-BP319982LQA
CSB-MP319982LQA
CSB-EP319982LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Probable membrane antigen 75 (75), partial CSB-YP319983SAX
CSB-EP319983SAX
CSB-BP319983SAX
CSB-MP319983SAX
CSB-EP319983SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Protein P (P), partial CSB-YP319984WAX
CSB-EP319984WAX
CSB-BP319984WAX
CSB-MP319984WAX
CSB-EP319984WAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opacity protein opA50 (opaC) CSB-YP319985NFZ
CSB-EP319985NFZ
CSB-BP319985NFZ
CSB-MP319985NFZ
CSB-EP319985NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E4 (E4) CSB-YP319986BKK
CSB-EP319986BKK
CSB-BP319986BKK
CSB-MP319986BKK
CSB-EP319986BKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Envelope protein US9 homolog, partial CSB-YP319987SRK1
CSB-EP319987SRK1
CSB-BP319987SRK1
CSB-MP319987SRK1
CSB-EP319987SRK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 3 Early E3 9.0 kDa glycoprotein, partial CSB-YP319988HIG1
CSB-EP319988HIG1
CSB-BP319988HIG1
CSB-MP319988HIG1
CSB-EP319988HIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Leucine--tRNA ligase, mitochondrial (NAM2), partial CSB-YP319989SVG
CSB-EP319989SVG
CSB-BP319989SVG
CSB-MP319989SVG
CSB-EP319989SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European elk papillomavirus Protein E5 (E5) CSB-YP319990EIN
CSB-EP319990EIN
CSB-BP319990EIN
CSB-MP319990EIN
CSB-EP319990EIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus 4 Replication-associated protein ORF2 (ORF2) CSB-YP319991SJY
CSB-EP319991SJY
CSB-BP319991SJY
CSB-MP319991SJY
CSB-EP319991SJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus 4 DNA binding protein ORF8 (ORF8) CSB-YP319992SJY
CSB-EP319992SJY
CSB-BP319992SJY
CSB-MP319992SJY
CSB-EP319992SJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Retrovirus-related Pol polyprotein LINE-1 (Pol), partial CSB-YP319993MO
CSB-EP319993MO
CSB-BP319993MO
CSB-MP319993MO
CSB-EP319993MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. R-phycocyanin-2 beta chain (rpcB) CSB-YP319994SSM
CSB-EP319994SSM
CSB-BP319994SSM
CSB-MP319994SSM
CSB-EP319994SSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vipera ammodytes ammodytes Phospholipase A2, ammodytoxin C (ATXC) CSB-YP319995VFO
CSB-EP319995VFO
CSB-BP319995VFO
CSB-MP319995VFO
CSB-EP319995VFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glucose-6-phosphate 1-dehydrogenase (ZWF1), partial CSB-YP319996SVG
CSB-EP319996SVG
CSB-BP319996SVG
CSB-MP319996SVG
CSB-EP319996SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila funebris Male accessory gland serine protease inhibitor (PapD) CSB-YP319997DGA
CSB-EP319997DGA
CSB-BP319997DGA
CSB-MP319997DGA
CSB-EP319997DGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aliphatic amidase (amiE) CSB-YP319998EZX
CSB-EP319998EZX
CSB-BP319998EZX
CSB-MP319998EZX
CSB-EP319998EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cyclin-dependent kinase 1 (Cdk1) CSB-YP319999MO
CSB-EP319999MO
CSB-BP319999MO
CSB-MP319999MO
CSB-EP319999MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Enterobactin synthase component F (entF), partial CSB-YP320000ENV
CSB-EP320000ENV
CSB-BP320000ENV
CSB-MP320000ENV
CSB-EP320000ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Quinolinate synthase A (nadA) CSB-YP320001ENV
CSB-EP320001ENV
CSB-BP320001ENV
CSB-MP320001ENV
CSB-EP320001ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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