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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica 30S ribosomal protein S19, chloroplastic (rps19-A) CSB-YP319369OFG
CSB-EP319369OFG
CSB-BP319369OFG
CSB-MP319369OFG
CSB-EP319369OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Protein psbN (psbN), partial CSB-YP319370MCB
CSB-EP319370MCB
CSB-BP319370MCB
CSB-MP319370MCB
CSB-EP319370MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Maturase K (matK), partial CSB-YP319371NHE
CSB-EP319371NHE
CSB-BP319371NHE
CSB-MP319371NHE
CSB-EP319371NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem I reaction center subunit VIII (psaI), partial CSB-YP319372MCB
CSB-EP319372MCB
CSB-BP319372MCB
CSB-MP319372MCB
CSB-EP319372MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem II reaction center protein J (psbJ), partial CSB-YP319373OFG
CSB-EP319373OFG
CSB-BP319373OFG
CSB-MP319373OFG
CSB-EP319373OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP319374MCB
CSB-EP319374MCB
CSB-BP319374MCB
CSB-MP319374MCB
CSB-EP319374MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem I assembly protein ycf3 (ycf3) CSB-YP319375MCB
CSB-EP319375MCB
CSB-BP319375MCB
CSB-MP319375MCB
CSB-EP319375MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP319376MCB
CSB-EP319376MCB
CSB-BP319376MCB
CSB-MP319376MCB
CSB-EP319376MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Putative membrane protein ycf1 N-terminal part (ycf1), partial CSB-YP319377MCB
CSB-EP319377MCB
CSB-BP319377MCB
CSB-MP319377MCB
CSB-EP319377MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum DNA-directed RNA polymerase subunit beta' (rpoC2), partial CSB-YP319378EWE
CSB-EP319378EWE
CSB-BP319378EWE
CSB-MP319378EWE
CSB-EP319378EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein D 1 (nodD1) CSB-YP319379BLH
CSB-EP319379BLH
CSB-BP319379BLH
CSB-MP319379BLH
CSB-EP319379BLH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein I (psbI), partial CSB-YP319380TNI
CSB-EP319380TNI
CSB-BP319380TNI
CSB-MP319380TNI
CSB-EP319380TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Thioredoxin-1 (trxA) CSB-YP319381FPY
CSB-EP319381FPY
CSB-BP319381FPY
CSB-MP319381FPY
CSB-EP319381FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS231B CSB-YP319382BCW
CSB-EP319382BCW
CSB-BP319382BCW
CSB-MP319382BCW
CSB-EP319382BCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Penicillin acylase CSB-YP319383LRU
CSB-EP319383LRU
CSB-BP319383LRU
CSB-MP319383LRU
CSB-EP319383LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Molybdopterin-synthase adenylyltransferase (moeB) CSB-YP319384ENV
CSB-EP319384ENV
CSB-BP319384ENV
CSB-MP319384ENV
CSB-EP319384ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein BlaR1 (blaR1), partial CSB-YP319385BQT1
CSB-EP319385BQT1
CSB-BP319385BQT1
CSB-MP319385BQT1
CSB-EP319385BQT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Glucose-1-phosphate adenylyltransferase large subunit (AGA.1) CSB-YP319386TQN
CSB-EP319386TQN
CSB-BP319386TQN
CSB-MP319386TQN
CSB-EP319386TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H1 (HHO) CSB-YP319387TLT
CSB-EP319387TLT
CSB-BP319387TLT
CSB-MP319387TLT
CSB-EP319387TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lathyrus sativus Mannose/glucose-specific lectin alpha chain CSB-YP319388LNR
CSB-EP319388LNR
CSB-BP319388LNR
CSB-MP319388LNR
CSB-EP319388LNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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