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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant cAMP-dependent protein kinase catalytic subunit (kin-1) CSB-YP321896CXY
CSB-EP321896CXY
CSB-BP321896CXY
CSB-MP321896CXY
CSB-EP321896CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhyparobia maderae Leucokinin-4 CSB-YP321897REJ
CSB-EP321897REJ
CSB-BP321897REJ
CSB-MP321897REJ
CSB-EP321897REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrococcus luteus 50S ribosomal protein L34 (rpmH) CSB-YP321898MTO
CSB-EP321898MTO
CSB-BP321898MTO
CSB-MP321898MTO
CSB-EP321898MTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP321899ENV
CSB-EP321899ENV
CSB-BP321899ENV
CSB-MP321899ENV
CSB-EP321899ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elizabethkingia miricola Peptide-N (4)- (N-acetyl-beta-D-glucosaminyl)asparagine amidase F CSB-YP321901EBK
CSB-EP321901EBK
CSB-BP321901EBK
CSB-MP321901EBK
CSB-EP321901EBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moorella thermoacetica Formate--tetrahydrofolate ligase (fhs), partial CSB-YP321902MUF
CSB-EP321902MUF
CSB-BP321902MUF
CSB-MP321902MUF
CSB-EP321902MUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrococcus luteus Ribonuclease P protein component (rnpA) CSB-YP321903MTO
CSB-EP321903MTO
CSB-BP321903MTO
CSB-MP321903MTO
CSB-EP321903MTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein (braC) CSB-YP321904EZX
CSB-EP321904EZX
CSB-BP321904EZX
CSB-MP321904EZX
CSB-EP321904EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Catalase HPII (katE) CSB-YP321905ENV
CSB-EP321905ENV
CSB-BP321905ENV
CSB-MP321905ENV
CSB-EP321905ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa A-type flagellin (fliC) CSB-YP321906EZW
CSB-EP321906EZW
CSB-BP321906EZW
CSB-MP321906EZW
CSB-EP321906EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Nucleoprotein (N), partial CSB-YP321907MJJ
CSB-EP321907MJJ
CSB-BP321907MJJ
CSB-MP321907MJJ
CSB-EP321907MJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA polymerase II (polB) CSB-YP321908ENV
CSB-BP321908ENV
CSB-MP321908ENV
CSB-EP321908ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Meiosis-inducing protein 1 (IME1) CSB-YP321909SVG
CSB-EP321909SVG
CSB-BP321909SVG
CSB-MP321909SVG
CSB-EP321909SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mordacia mordax Globin-3 CSB-YP321910MIH
CSB-EP321910MIH
CSB-BP321910MIH
CSB-MP321910MIH
CSB-EP321910MIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein H (lcrH) CSB-YP321911YAS
CSB-EP321911YAS
CSB-BP321911YAS
CSB-MP321911YAS
CSB-EP321911YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Soluble inorganic pyrophosphatase 2 (PPA2) CSB-YP321912DOA
CSB-EP321912DOA
CSB-BP321912DOA
CSB-MP321912DOA
CSB-EP321912DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant High-molecular weight cobalt-containing nitrile hydratase subunit alpha (nhhA) CSB-YP321913RDH
CSB-EP321913RDH
CSB-BP321913RDH
CSB-MP321913RDH
CSB-EP321913RDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized leukocidin-like protein 1 (SACOL2004) CSB-YP321914FLB
CSB-EP321914FLB
CSB-BP321914FLB
CSB-MP321914FLB
CSB-EP321914FLB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Regulatory protein alcR (alcR), partial CSB-YP321915EKF
CSB-EP321915EKF
CSB-BP321915EKF
CSB-MP321915EKF
CSB-EP321915EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA methylase leader peptide CSB-YP321916CQF
CSB-EP321916CQF
CSB-BP321916CQF
CSB-MP321916CQF
CSB-EP321916CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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