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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Transcriptional activator protein CUP2 (CUP2) CSB-YP322767SVG
CSB-EP322767SVG
CSB-BP322767SVG
CSB-MP322767SVG
CSB-EP322767SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase vanadium-iron protein beta chain (vnfK) CSB-YP322768DPU
CSB-EP322768DPU
CSB-BP322768DPU
CSB-MP322768DPU
CSB-EP322768DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Sperm histone P2a CSB-YP322769HO
CSB-EP322769HO
CSB-BP322769HO
CSB-MP322769HO
CSB-EP322769HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halococcus morrhuae 30S ribosomal protein S7P (rps7p) CSB-YP322770HBP
CSB-EP322770HBP
CSB-BP322770HBP
CSB-MP322770HBP
CSB-EP322770HBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Allantoate permease (DAL5), partial CSB-YP322771SVG
CSB-EP322771SVG
CSB-BP322771SVG
CSB-MP322771SVG
CSB-EP322771SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Levansucrase and sucrase synthesis operon antiterminator (sacY) CSB-YP322772BRJ
CSB-EP322772BRJ
CSB-BP322772BRJ
CSB-MP322772BRJ
CSB-EP322772BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Bombyxin C-1 (BBXC1) CSB-YP322773BTT
CSB-EP322773BTT
CSB-BP322773BTT
CSB-MP322773BTT
CSB-EP322773BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage I2-2 Virion export protein (IV), partial CSB-YP322774ECP1
CSB-EP322774ECP1
CSB-BP322774ECP1
CSB-MP322774ECP1
CSB-EP322774ECP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 2S seed storage protein 2 (AT2S2) CSB-YP322775DOA
CSB-EP322775DOA
CSB-BP322775DOA
CSB-MP322775DOA
CSB-EP322775DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psophocarpus tetragonolobus Albumin-1 CSB-YP322776FGZ
CSB-EP322776FGZ
CSB-BP322776FGZ
CSB-MP322776FGZ
CSB-EP322776FGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus borealis Actin, cytoplasmic 1 (actb) CSB-YP322777XAO
CSB-EP322777XAO
CSB-BP322777XAO
CSB-MP322777XAO
CSB-EP322777XAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Excisionase (xis) CSB-YP322778SUV
CSB-EP322778SUV
CSB-BP322778SUV
CSB-MP322778SUV
CSB-EP322778SUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Hemolysin secretion protein (hlyB), partial CSB-YP322779VEX1
CSB-EP322779VEX1
CSB-BP322779VEX1
CSB-MP322779VEX1
CSB-EP322779VEX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Juvenile hormone-binding protein (JHBP) CSB-YP322780MPV
CSB-EP322780MPV
CSB-BP322780MPV
CSB-MP322780MPV
CSB-EP322780MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Morphine-modulating neuropeptide B CSB-YP322781BO
CSB-EP322781BO
CSB-BP322781BO
CSB-MP322781BO
CSB-EP322781BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteriochlorophyll c-binding protein (csmA) CSB-YP322782CJL
CSB-EP322782CJL
CSB-BP322782CJL
CSB-MP322782CJL
CSB-EP322782CJL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Uncharacterized 49.1 kDa protein in ND3 intron CSB-YP322783EXJ
CSB-EP322783EXJ
CSB-BP322783EXJ
CSB-MP322783EXJ
CSB-EP322783EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytophthora cryptogea Beta-elicitin cryptogein CSB-YP322784PJL
CSB-BP322784PJL
CSB-MP322784PJL
CSB-EP322784PJL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 10 kDa chaperonin (groS) CSB-YP322785DSL
CSB-EP322785DSL
CSB-BP322785DSL
CSB-MP322785DSL
CSB-EP322785DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized mitochondrial protein ORF2 CSB-YP322786ERW
CSB-EP322786ERW
CSB-BP322786ERW
CSB-MP322786ERW
CSB-EP322786ERW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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