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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bungarus multicinctus Kappa-2-bungarotoxin CSB-YP323387BXN
CSB-EP323387BXN
CSB-BP323387BXN
CSB-MP323387BXN
CSB-EP323387BXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP323388HMD
CSB-EP323388HMD
CSB-BP323388HMD
CSB-MP323388HMD
CSB-EP323388HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichostrongylus colubriformis Tropomyosin, muscle CSB-YP323389TQF
CSB-EP323389TQF
CSB-BP323389TQF
CSB-MP323389TQF
CSB-EP323389TQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Homeobox protein abdominal-A homolog (ABD-A) CSB-YP323390DNK
CSB-EP323390DNK
CSB-BP323390DNK
CSB-MP323390DNK
CSB-EP323390DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum D phage Mono-ADP-ribosyltransferase C3 (C3) CSB-YP323391CLR
CSB-EP323391CLR
CSB-BP323391CLR
CSB-MP323391CLR
CSB-EP323391CLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Oxygen-insensitive NAD (P)H nitroreductase CSB-YP323392SXB
CSB-EP323392SXB
CSB-BP323392SXB
CSB-MP323392SXB
CSB-EP323392SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-R8A2 B Uncharacterized protein ORF5 (ORF5), partial CSB-YP323393SJZ1
CSB-EP323393SJZ1
CSB-BP323393SJZ1
CSB-MP323393SJZ1
CSB-EP323393SJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa Protochlorophyllide reductase CSB-YP323394DPO
CSB-EP323394DPO
CSB-BP323394DPO
CSB-MP323394DPO
CSB-EP323394DPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Protein FPV129 (FPV129), partial CSB-YP323395FEY1
CSB-EP323395FEY1
CSB-BP323395FEY1
CSB-MP323395FEY1
CSB-EP323395FEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exo-poly-alpha-D-galacturonosidase (pehX), partial CSB-YP323396EGI
CSB-EP323396EGI
CSB-BP323396EGI
CSB-MP323396EGI
CSB-EP323396EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar M-ring protein (fliF), partial CSB-YP323397SXB1
CSB-EP323397SXB1
CSB-BP323397SXB1
CSB-MP323397SXB1
CSB-EP323397SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Acetyl-CoA hydrolase (acu-8), partial CSB-YP323398NHA
CSB-EP323398NHA
CSB-BP323398NHA
CSB-MP323398NHA
CSB-EP323398NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kallikrein 1-related peptidase b9 (Klk1b9) CSB-YP323399MO
CSB-EP323399MO
CSB-BP323399MO
CSB-MP323399MO
CSB-EP323399MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1), partial CSB-YP323400TGA
CSB-EP323400TGA
CSB-BP323400TGA
CSB-MP323400TGA
CSB-EP323400TGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative per-hexamer repeat protein 2 (Phxr2) CSB-YP323401MO
CSB-EP323401MO
CSB-BP323401MO
CSB-MP323401MO
CSB-EP323401MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Class I histocompatibility antigen, Non-RT1.A alpha-1 chain (RT1-Aw2), partial CSB-YP323402RA
CSB-EP323402RA
CSB-BP323402RA
CSB-MP323402RA
CSB-EP323402RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans ATP synthase subunit 9, mitochondrial (atp9), partial CSB-YP323403EKF1
CSB-EP323403EKF1
CSB-BP323403EKF1
CSB-MP323403EKF1
CSB-EP323403EKF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Protein moxJ (moxJ) CSB-YP323404MSZ
CSB-EP323404MSZ
CSB-BP323404MSZ
CSB-MP323404MSZ
CSB-EP323404MSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian cytomegalovirus Protease precursor (UL80), partial CSB-YP323405SGO
CSB-EP323405SGO
CSB-BP323405SGO
CSB-MP323405SGO
CSB-EP323405SGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus longicaudatus Guanine nucleotide-binding protein G (s) subunit alpha CSB-YP323406DXV
CSB-EP323406DXV
CSB-BP323406DXV
CSB-MP323406DXV
CSB-EP323406DXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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