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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP321957IAZ1
CSB-EP321957IAZ1
CSB-BP321957IAZ1
CSB-MP321957IAZ1
CSB-EP321957IAZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana debneyi 50S ribosomal protein L2, chloroplastic (rpl2) CSB-YP321958NDI
CSB-EP321958NDI
CSB-BP321958NDI
CSB-MP321958NDI
CSB-EP321958NDI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline leukemia virus Envelope glycoprotein (env) CSB-YP321959FAO
CSB-EP321959FAO
CSB-BP321959FAO
CSB-MP321959FAO
CSB-EP321959FAO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline sarcoma virus Envelope glycoprotein (env), partial CSB-YP321960FBB
CSB-EP321960FBB
CSB-BP321960FBB
CSB-MP321960FBB
CSB-EP321960FBB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Multidrug resistance protein 2 (PGY2), partial CSB-YP321961DXU
CSB-EP321961DXU
CSB-BP321961DXU
CSB-MP321961DXU
CSB-EP321961DXU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 28.1 kDa virulence protein (mkaB) CSB-YP321962SWX
CSB-EP321962SWX
CSB-BP321962SWX
CSB-MP321962SWX
CSB-EP321962SWX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase sina (sina) CSB-YP321963DLU
CSB-EP321963DLU
CSB-BP321963DLU
CSB-MP321963DLU
CSB-EP321963DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S2 (rpsB) CSB-YP321964BRJ
CSB-EP321964BRJ
CSB-BP321964BRJ
CSB-MP321964BRJ
CSB-EP321964BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S5 (rpsE) CSB-YP321965BRJ
CSB-EP321965BRJ
CSB-BP321965BRJ
CSB-MP321965BRJ
CSB-EP321965BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S9 (rpsI) CSB-YP321966BRJ
CSB-EP321966BRJ
CSB-BP321966BRJ
CSB-MP321966BRJ
CSB-EP321966BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S15 (rpsO) CSB-YP321967BRJ
CSB-EP321967BRJ
CSB-BP321967BRJ
CSB-MP321967BRJ
CSB-EP321967BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S19 (rpsS) CSB-YP321968BRJ
CSB-EP321968BRJ
CSB-BP321968BRJ
CSB-MP321968BRJ
CSB-EP321968BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L2P (rpl2p) CSB-YP321969MEZ
CSB-EP321969MEZ
CSB-BP321969MEZ
CSB-MP321969MEZ
CSB-EP321969MEZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease R (rnr), partial CSB-YP321970ENV
CSB-EP321970ENV
CSB-BP321970ENV
CSB-MP321970ENV
CSB-EP321970ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S6e (rps6e) CSB-YP321971HTJ
CSB-EP321971HTJ
CSB-BP321971HTJ
CSB-MP321971HTJ
CSB-EP321971HTJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP321972EHU
CSB-EP321972EHU
CSB-BP321972EHU
CSB-MP321972EHU
CSB-EP321972EHU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acyl carrier protein phosphodiesterase (acpH) CSB-YP321973ENV
CSB-BP321973ENV
CSB-MP321973ENV
CSB-EP321973ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hirudo medicinalis Homeobox protein LOX2 (LOX2) CSB-YP321974HSM
CSB-EP321974HSM
CSB-BP321974HSM
CSB-MP321974HSM
CSB-EP321974HSM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribonucleoside-diphosphate reductase large chain 1 (RNR1), partial CSB-YP321975SVG
CSB-EP321975SVG
CSB-BP321975SVG
CSB-MP321975SVG
CSB-EP321975SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP321976IFG
CSB-EP321976IFG
CSB-BP321976IFG
CSB-MP321976IFG
CSB-EP321976IFG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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